Rapid assignment of protein side chain resonances using projection-reconstruction of (4,3)D HC(CCO)NH and intra-HC(C)NH experiments.

Journal Article (Journal Article)

The reconstruction of higher-dimensional NMR spectra from projections can provide significant savings in instrument time. Here, we demonstrate its application to the (4,3)D HC(CCO)NH and intra-HC(C)NH experiments. The latter experiment contains a novel intra-residue filter element, which selectively correlates the side chain resonances with the corresponding intra-residue amide resonances. Compared to the conventional HC(C)NH experiment, the intra-HC(C)NH experiment reduces the spectral complexity and thus the minimum number of projections required for artifact-free reconstruction by half. The use of the projection-reconstruction technique allows rapid data collection and unambiguous assignment of aliphatic side chain nuclei at high resolution.

Full Text

Duke Authors

Cited Authors

  • Jiang, L; Coggins, BE; Zhou, P

Published Date

  • July 2005

Published In

Volume / Issue

  • 175 / 1

Start / End Page

  • 170 - 176

PubMed ID

  • 15949755

International Standard Serial Number (ISSN)

  • 1090-7807

Digital Object Identifier (DOI)

  • 10.1016/j.jmr.2005.03.014


  • eng

Conference Location

  • United States