(4,2)D Projection--reconstruction experiments for protein backbone assignment: application to human carbonic anhydrase II and calbindin D(28K).

Published

Journal Article

Projection-reconstruction NMR experiments have been shown to significantly reduce the acquisition time required to obtain protein backbone assignment data. To date, this concept has only been applied to smaller (15)N/(13)C-labeled proteins. Here, we show that projection-reconstruction NMR techniques can be extended to larger protonated and perdeuterated proteins. We present a suite of (4,2)D triple-resonance experiments for protein backbone assignment and a Hybrid Backprojection/Lower-Value algorithm for reconstructing data with relatively weak signal-to-noise ratios. In addition, we propose a sampling theorem and discuss its implication on the choice of projection angles. We demonstrate the efficacy of this approach using the 29 kDa protein, human carbonic anhydrase II and the 30 kDa protein, calbindin D(28K).

Full Text

Duke Authors

Cited Authors

  • Venters, RA; Coggins, BE; Kojetin, D; Cavanagh, J; Zhou, P

Published Date

  • June 22, 2005

Published In

Volume / Issue

  • 127 / 24

Start / End Page

  • 8785 - 8795

PubMed ID

  • 15954785

Pubmed Central ID

  • 15954785

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja0509580

Language

  • eng

Conference Location

  • United States