Biological activity of interleukin-10 in the central nervous system.
Journal Article
Cytokines play a crucial role as mediators of inflammation. Astrocytes and microglia are the two major glial cells involved in the central nervous system immune responses. In this study we examined the effects of interleukin-10 (IL-10), one of the naturally occurring inhibitory cytokines, on different types of glial cells in culture such as rat astrocytes, hamster microglia and C6-2B glioma cells. Phosphorylation of signal transducers and activators of transcription (STAT) proteins was used as a marker for IL-10 activity. Within minutes, IL-10 elicited a strong and weak increase in STAT3 and STAT1 phosphorylation, respectively, in human T lymphocytes, suggesting that STAT3 is a main IL-10 signaling event in these cells. In contrast, IL-10 failed to induce STAT3 in glial cells, but elicited a weak increase in STAT1 phosphorylation in microglia and C6-2B glioma cells only, suggesting that in some glial cell population(s) IL-10 may produce cellular responses via activation of the STAT1 pathway. Moreover, in C6-2B cells, IL-10 elicited a decrease in the level of basic fibroblast growth factor mRNA. A similar decrease was observed in adult rat hypothalamus, indicating that this cytokine may regulate glial production of trophic factors. Our data suggest that IL-10 may play a role in glial cell differentiation and proliferation.
Full Text
Duke Authors
Cited Authors
- Zocchia, C; Spiga, G; Rabin, SJ; Grekova, M; Richert, J; Chernyshev, O; Colton, C; Mocchetti, I
Published Date
- April 1997
Published In
Volume / Issue
- 30 / 4-5
Start / End Page
- 433 - 439
PubMed ID
- 9106258
Pubmed Central ID
- 9106258
International Standard Serial Number (ISSN)
- 0197-0186
Digital Object Identifier (DOI)
- 10.1016/s0197-0186(96)00079-4
Language
- eng
Conference Location
- England