Mitogenic effect of neuropeptide Y in rat vascular smooth muscle cells.

Published

Journal Article

Neuropeptide Y (NPY) is a vasoconstrictor released with norepinephrine from perivascular sympathetic nerves. Since sympathetic nerves appear to play a role in vascular smooth muscle cell (SMC) hypertrophy, we studied the effects of NPY on proliferation of cultured rat aorta- and vena cava-derived SMC. Both cell types displayed high-affinity NPY binding sites with displacement characteristics of [Pro34]NPY > NPY(13-36) > NPY(18-36) in aorta and [Pro34]NPY = NPY(13-36) = NPY(18-36) in the vena cava. Incubation with NPY (50-1000 nM) for 48 h increased by up to twofold cell number and [3H]-thymidine incorporation in both cell types (aortic more sensitive to NPY than venous). Following incubation with NPY, the disappearance of NPY immunoreactivity (-IR) from media was markedly delayed in the presence of SMC, and cell content of NPY-IR increased in a dose-dependent manner, indicating that SMC either diminish degradation of the peptide (possibly by internalization) or secrete endogenous NPY (or both). Structure-activity relationship studies with NPY(18-36) indicated involvement of Y1 receptors in mitogenesis. Thus, NPY has a mitogenic effect (probably mediated by Y1 receptors) and, therefore, may be a sympathetic trophic factor involved in vascular hypertrophy.

Full Text

Duke Authors

Cited Authors

  • Zukowska-Grojec, Z; Pruszczyk, P; Colton, C; Yao, J; Shen, GH; Myers, AK; Wahlestedt, C

Published Date

  • March 1, 1993

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 263 - 268

PubMed ID

  • 8483806

Pubmed Central ID

  • 8483806

International Standard Serial Number (ISSN)

  • 0196-9781

Language

  • eng

Conference Location

  • United States