Creating porcine biomedical models through recombineering.

Published

Journal Article

Recent advances in genomics provide genetic information from humans and other mammals (mouse, rat, dog and primates) traditionally used as models as well as new candidates (pigs and cattle). In addition, linked enabling technologies, such as transgenesis and animal cloning, provide innovative ways to design and perform experiments to dissect complex biological systems. Exploitation of genomic information overcomes the traditional need to choose naturally occurring models. Thus, investigators can utilize emerging genomic knowledge and tools to create relevant animal models. This approach is referred to as reverse genetics. In contrast to 'forward genetics', in which gene(s) responsible for a particular phenotype are identified by positional cloning (phenotype to genotype), the 'reverse genetics' approach determines the function of a gene and predicts the phenotype of a cell, tissue, or organism (genotype to phenotype). The convergence of classical and reverse genetics, along with genomics, provides a working definition of a 'genetic model' organism (3). The recent construction of phenotypic maps defining quantitative trait loci (QTL) in various domesticated species provides insights into how allelic variations contribute to phenotypic diversity. Targeted chromosomal regions are characterized by the construction of bacterial artificial chromosome (BAC) contigs to isolate and characterize genes contributing towards phenotypic variation. Recombineering provides a powerful methodology to harvest genetic information responsible for phenotype. Linking recombineering with gene-targeted homologous recombination, coupled with nuclear transfer (NT) technology can provide 'clones' of genetically modified animals.

Full Text

Duke Authors

Cited Authors

  • Rogatcheva, MM; Rund, LA; Swanson, KS; Marron, BM; Beever, JE; Counter, CM; Schook, LB

Published Date

  • 2004

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • 262 - 267

PubMed ID

  • 18629152

Pubmed Central ID

  • 18629152

International Standard Serial Number (ISSN)

  • 1531-6912

Digital Object Identifier (DOI)

  • 10.1002/cfg.404

Language

  • eng

Conference Location

  • Egypt