Skip to main content
Journal cover image

Estrogen receptor beta protects against in vivo injury in RPE cells.

Publication ,  Journal Article
Elliot, SJ; Catanuto, P; Espinosa-Heidmann, DG; Fernandez, P; Hernandez, E; Saloupis, P; Korach, K; Karl, M; Cousins, SW
Published in: Exp Eye Res
January 2010

Epidemiological data suggest that estrogen deficiency in postmenopausal women may contribute to the severity of AMD. We discovered that 17beta-estradiol (E2) was a crucial regulator of the severity of extracellular matrix turnover (ECM) dysregulation both in vivo and in vitro. We also found in vitro that the presence of estrogen receptor (ER)beta regulates MMP-2 activity. Therefore in an attempt to delineate the role of the ER subtypes, female estrogen receptor knockout (ERKO) mice were fed a high-fat diet, and the eyes were exposed to seven 5-second doses of nonphototoxic levels of blue-green light over 2 weeks. Three months after cessation of blue light treatment, transmission electron microscopy was performed to assess severity of deposits, Bruchs membrane changes, and choriocapillaris endothelial morphology. We found that changes in the trimolecular complex of pro-MMP-2, MMP-14 and TIMP-2 correlated with increased Bruch's membrane thickening or sub-retinal deposit formation (basal laminar deposits) in ERKObeta mice. In addition RPE isolated from ERKObeta mice had an increase in expression of total collagen and a decrease in MMP-2 activity. Finally we found that ERK an intermediate signaling molecule in the MMP pathway was activated in RPE isolated from ERKObeta mice. These data suggest that mice which lack ERbeta are more susceptible to in vivo injury associated with environmental light and high fat diet.

Duke Scholars

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

January 2010

Volume

90

Issue

1

Start / End Page

10 / 16

Location

England

Related Subject Headings

  • Tissue Inhibitor of Metalloproteinase-2
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Pigment Epithelium
  • Oxidative Stress
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Microscopy, Electron, Transmission
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Elliot, S. J., Catanuto, P., Espinosa-Heidmann, D. G., Fernandez, P., Hernandez, E., Saloupis, P., … Cousins, S. W. (2010). Estrogen receptor beta protects against in vivo injury in RPE cells. Exp Eye Res, 90(1), 10–16. https://doi.org/10.1016/j.exer.2009.09.001
Elliot, Sharon J., Paola Catanuto, Diego G. Espinosa-Heidmann, Pedro Fernandez, Eleut Hernandez, Peter Saloupis, Kenneth Korach, Michael Karl, and Scott W. Cousins. “Estrogen receptor beta protects against in vivo injury in RPE cells.Exp Eye Res 90, no. 1 (January 2010): 10–16. https://doi.org/10.1016/j.exer.2009.09.001.
Elliot SJ, Catanuto P, Espinosa-Heidmann DG, Fernandez P, Hernandez E, Saloupis P, et al. Estrogen receptor beta protects against in vivo injury in RPE cells. Exp Eye Res. 2010 Jan;90(1):10–6.
Elliot, Sharon J., et al. “Estrogen receptor beta protects against in vivo injury in RPE cells.Exp Eye Res, vol. 90, no. 1, Jan. 2010, pp. 10–16. Pubmed, doi:10.1016/j.exer.2009.09.001.
Elliot SJ, Catanuto P, Espinosa-Heidmann DG, Fernandez P, Hernandez E, Saloupis P, Korach K, Karl M, Cousins SW. Estrogen receptor beta protects against in vivo injury in RPE cells. Exp Eye Res. 2010 Jan;90(1):10–16.
Journal cover image

Published In

Exp Eye Res

DOI

EISSN

1096-0007

Publication Date

January 2010

Volume

90

Issue

1

Start / End Page

10 / 16

Location

England

Related Subject Headings

  • Tissue Inhibitor of Metalloproteinase-2
  • Reverse Transcriptase Polymerase Chain Reaction
  • Retinal Pigment Epithelium
  • Oxidative Stress
  • Ophthalmology & Optometry
  • Oligonucleotide Array Sequence Analysis
  • Microscopy, Electron, Transmission
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Mice