DNA strand breaks following in vitro exposure to asbestos increase with surface-complexed [Fe3+].

Published

Journal Article

Surface functional groups on silicate dusts complex iron cations which can cycle through reduction and oxidation states to generate free radicals. These oxidants have a capacity to produce DNA strand breaks and mutations which are primary events in cancer induction. A differential in the capacity of fibrous silicates to produce carcinoma is recognized with the amphiboles demonstrating a greater biologic effect than the serpentine fiber chrysotile. We tested the hypothesis that the differences in genotoxicity of these fibrous silicates correspond to varying concentrations of iron complexed to the surface. Relative to chrysotile, the amphibole fibers complexed greater amounts of iron cations from both inorganic and in vivo sources. Increased concentrations of surface-complexed iron were associated with greater oxidant generation, measured as thiobarbituric acid-reactive products of deoxyribose, and more covalently closed, circular DNA strand scission. These results indicate that genotoxic effects of these fibers may correspond to their capacity to complex iron at the surface.

Full Text

Duke Authors

Cited Authors

  • Ghio, AJ; Kennedy, TP; Stonehuerner, JG; Crumbliss, AL; Hoidal, JR

Published Date

  • May 1994

Published In

Volume / Issue

  • 311 / 1

Start / End Page

  • 13 - 18

PubMed ID

  • 8185309

Pubmed Central ID

  • 8185309

Electronic International Standard Serial Number (EISSN)

  • 1096-0384

International Standard Serial Number (ISSN)

  • 0003-9861

Digital Object Identifier (DOI)

  • 10.1006/abbi.1994.1202

Language

  • eng