In vivo hypermutation of xenotropic murine leukemia virus-related virus DNA in peripheral blood mononuclear cells of rhesus macaque by APOBEC3 proteins.

Published

Journal Article

The gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), replicates to high titers in some human cell lines and is able to infect non-human primates. To determine whether APOBEC3 (A3) proteins restrict XMRV infections in a non-human primate model, we sequenced proviral DNA from peripheral blood mononuclear cells of XMRV-infected rhesus macaques. Hypermutation characteristic of A3DE, A3F and A3G activities was observed in the XMRV proviral sequences in vivo. Furthermore, expression of rhesus A3DE, A3F, or A3G in human cells inhibited XMRV infection and caused hypermutation of XMRV DNA. These studies show that some rhesus A3 isoforms are highly effective against XMRV in the blood of a non-human primate model of infection and in cultured human cells.

Full Text

Duke Authors

Cited Authors

  • Zhang, A; Bogerd, H; Villinger, F; Das Gupta, J; Dong, B; Klein, EA; Hackett, J; Schochetman, G; Cullen, BR; Silverman, RH

Published Date

  • December 5, 2011

Published In

Volume / Issue

  • 421 / 1

Start / End Page

  • 28 - 33

PubMed ID

  • 21982221

Pubmed Central ID

  • 21982221

Electronic International Standard Serial Number (EISSN)

  • 1096-0341

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2011.08.030

Language

  • eng

Conference Location

  • United States