Viral microRNA targetome of KSHV-infected primary effusion lymphoma cell lines.

Published

Journal Article

Primary effusion lymphoma (PEL) is caused by Kaposi's sarcoma-associated herpesvirus (KSHV) and frequently also harbors Epstein-Barr virus (EBV). The expression of KSHV- and EBV-encoded microRNAs (miRNAs) in PELs suggests a role for these miRNAs in latency and lymphomagenesis. Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites. In addition to a known viral analog of cellular miR-155, we show that KSHV encodes a viral miRNA that mimics cellular miR-142-3p function. In summary, this study identifies an extensive list of KSHV miRNA targets, which are likely to influence viral replication and pathogenesis.

Full Text

Duke Authors

Cited Authors

  • Gottwein, E; Corcoran, DL; Mukherjee, N; Skalsky, RL; Hafner, M; Nusbaum, JD; Shamulailatpam, P; Love, CL; Dave, SS; Tuschl, T; Ohler, U; Cullen, BR

Published Date

  • November 17, 2011

Published In

Volume / Issue

  • 10 / 5

Start / End Page

  • 515 - 526

PubMed ID

  • 22100165

Pubmed Central ID

  • 22100165

Electronic International Standard Serial Number (EISSN)

  • 1934-6069

Digital Object Identifier (DOI)

  • 10.1016/j.chom.2011.09.012

Language

  • eng

Conference Location

  • United States