Analysis of rhesus rhadinovirus microRNAs expressed in virus-induced tumors from infected rhesus macaques.
Journal Article (Journal Article)
Rhesus rhadinovirus (RRV), a primate gamma-herpesvirus related to human Kaposi's sarcoma-associated herpesvirus (KSHV), causes a similar pattern of pathogenesis. Previously, RRV was shown to express 7 pre-microRNAs (pre-miRNAs) in latently infected cells. Using deep sequencing, we analyzed the pattern of small RNA expression in vivo using latently RRV-infected B-cell lymphoma and retroperitoneal fibromatosis tissues. We identified 15 virally encoded pre-miRNAs in both tumors, including all previously reported RRV pre-miRNAs. Although all 15 RRV pre-miRNAs, like all 12 KSHV pre-miRNAs, are located 3' to the conserved viral ORF71 gene and in the same transcriptional orientation, only one RRV miRNA is homologous to a KSHV miRNA. One previously identified RRV miRNA, miR-rR1-3, is actually a miRNA offset RNA (moRNA) derived from sequences located adjacent to pre-miR-rR1-3. Several other RRV-derived moRNAs were obtained, including one recovered >600 times. Together, this research provides a comprehensive list of the miRNAs and moRNAs encoded by RRV.
Full Text
Duke Authors
Cited Authors
- Umbach, JL; Strelow, LI; Wong, SW; Cullen, BR
Published Date
- September 30, 2010
Published In
Volume / Issue
- 405 / 2
Start / End Page
- 592 - 599
PubMed ID
- 20655562
Pubmed Central ID
- PMC2923253
Electronic International Standard Serial Number (EISSN)
- 1096-0341
Digital Object Identifier (DOI)
- 10.1016/j.virol.2010.06.036
Language
- eng
Conference Location
- United States