Identification of viral microRNAs expressed in human sacral ganglia latently infected with herpes simplex virus 2.


Journal Article

Deep sequencing of small RNAs isolated from human sacral ganglia latently infected with herpes simplex virus 2 (HSV-2) was used to identify HSV-2 microRNAs (miRNAs) expressed during latent infection. This effort resulted in the identification of five distinct HSV-2 miRNA species, two of which, miR-H3/miR-I and miR-H4/miR-II, have been previously reported. Three novel HSV-2 miRNAs were also identified, and two of these, miR-H7 and miR-H9, are derived from the latency-associated transcript (LAT) and are located antisense to the viral transcript encoding transactivator ICP0. A third novel HSV-2 miRNA, miR-H10, is encoded within the unique long (U(L)) region of the genome, 3' to the U(L)15 open reading frame, and is presumably excised from a novel, latent HSV-2 transcript distinct from LAT.

Full Text

Duke Authors

Cited Authors

  • Umbach, JL; Wang, K; Tang, S; Krause, PR; Mont, EK; Cohen, JI; Cullen, BR

Published Date

  • January 2010

Published In

Volume / Issue

  • 84 / 2

Start / End Page

  • 1189 - 1192

PubMed ID

  • 19889786

Pubmed Central ID

  • 19889786

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

Digital Object Identifier (DOI)

  • 10.1128/JVI.01712-09


  • eng

Conference Location

  • United States