Biochemical and functional analysis of soluble human interleukin-2 receptor produced in rodent cells. Solid-phase reconstitution of a receptor-ligand binding reaction.

Published

Journal Article

The binding of interleukin-2 (IL-2) to the IL-2 receptor (IL-2R) on human T-cells is a key regulatory event which is absolutely required for T-cell-mediated immune responses. To understand further this binding event, we modified the human IL-2R gene to encode a secreted form of IL-2R. Secreted IL-2R was then expressed at very high levels (approximately 11 micrograms/10(6) cells/48 h) in rodent cells using gene-linked co-amplification. The soluble forms of IL-2R were shown to retain IL-2 affinity shown by cell-surface IL-2R (Kd approximately 18 nM) and were purified to homogeneity using IL-2 affinity chromatography. Purified, recombinant IL-2R and biotinylated IL-2 were used to establish a solid-phase receptor binding assay. Binding of IL-2-biotin was demonstrated to be dose-dependent at concentrations ranging from 10 to 1000 ng/ml, and the specificity of receptor-ligand binding was demonstrated by competition with non-biotinylated IL-2 and with anti-receptor antibodies known to block IL-2 binding in vivo. This immunosorbent receptor assay offers a simple and rapid method for studying the binding of IL-2 to human IL-2R.

Full Text

Duke Authors

Cited Authors

  • Hakimi, J; Seals, C; Anderson, LE; Podlaski, FJ; Lin, P; Danho, W; Jenson, JC; Perkins, A; Donadio, PE; Familletti, PC

Published Date

  • December 25, 1987

Published In

Volume / Issue

  • 262 / 36

Start / End Page

  • 17336 - 17341

PubMed ID

  • 3121594

Pubmed Central ID

  • 3121594

International Standard Serial Number (ISSN)

  • 0021-9258

Language

  • eng

Conference Location

  • United States