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Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden.

Publication ,  Journal Article
Hoang, CD; D'Cunha, J; Tawfic, SH; Gruessner, AC; Kratzke, RA; Maddaus, MA
Published in: J Thorac Cardiovasc Surg
May 2004

OBJECTIVE: This study hypothesized that non-small cell lung carcinoma cells from primary tumors isolated by laser capture microdissection would exhibit gene expression profiles associated with graded lymph node metastatic cell burden. METHODS: Non-small cell lung carcinoma tumors (n = 15) were classified on the basis of nodal metastatic cell burden by 2 methods, obtaining 3 groups: no metastasis, micrometastasis, and overt metastasis. We then performed microarray analysis on microdissected primary tumor cells and identified gene expression profiles associated with graded nodal tumor burden using a correlation-based selection algorithm coupled with cross-validation analysis. Hierarchical clustering showed the regrouping of tumor specimens; the classification inference was assessed with Fisher's exact test. We verified data for certain genes by using another independent assay. RESULTS: The 15 specimens clustered into 3 groups: cluster A predominated in specimens with overt nodal metastasis; cluster B had more specimens with nodal micrometastases; and cluster C included only specimens without nodal metastases. Cluster assignment was based on a validated 75-gene discriminatory subset. Notably, genes not previously associated with positive non-small cell lung carcinoma lymph node status were encountered in the profiling analysis. CONCLUSIONS: Microdissection, combined with microarray analysis, is a potentially powerful method to characterize the molecular profile of tumor cells. The 75-gene expression profiles representative of clusters A and B may define genotypes prone to metastasize. Overall, the 3 groups of tumor specimens clustered separately, suggesting that this approach may identify graded metastatic propensity. Further, genes singled out in clustering may yield insights into underlying metastatic mechanisms and may represent new therapeutic targets.

Duke Scholars

Published In

J Thorac Cardiovasc Surg

DOI

ISSN

0022-5223

Publication Date

May 2004

Volume

127

Issue

5

Start / End Page

1332 / 1341

Location

United States

Related Subject Headings

  • Survival Rate
  • Respiratory System
  • RNA, Neoplasm
  • Oligonucleotide Array Sequence Analysis
  • Lymphatic Metastasis
  • Lymph Nodes
  • Lung Neoplasms
  • Humans
  • Genotype
  • Gene Expression Profiling
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hoang, C. D., D’Cunha, J., Tawfic, S. H., Gruessner, A. C., Kratzke, R. A., & Maddaus, M. A. (2004). Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden. J Thorac Cardiovasc Surg, 127(5), 1332–1341. https://doi.org/10.1016/j.jtcvs.2003.11.060
Hoang, Chuong D., Jonathan D’Cunha, Sherif H. Tawfic, Angelika C. Gruessner, Robert A. Kratzke, and Michael A. Maddaus. “Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden.J Thorac Cardiovasc Surg 127, no. 5 (May 2004): 1332–41. https://doi.org/10.1016/j.jtcvs.2003.11.060.
Hoang CD, D’Cunha J, Tawfic SH, Gruessner AC, Kratzke RA, Maddaus MA. Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden. J Thorac Cardiovasc Surg. 2004 May;127(5):1332–41.
Hoang, Chuong D., et al. “Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden.J Thorac Cardiovasc Surg, vol. 127, no. 5, May 2004, pp. 1332–41. Pubmed, doi:10.1016/j.jtcvs.2003.11.060.
Hoang CD, D’Cunha J, Tawfic SH, Gruessner AC, Kratzke RA, Maddaus MA. Expression profiling of non-small cell lung carcinoma identifies metastatic genotypes based on lymph node tumor burden. J Thorac Cardiovasc Surg. 2004 May;127(5):1332–1341.
Journal cover image

Published In

J Thorac Cardiovasc Surg

DOI

ISSN

0022-5223

Publication Date

May 2004

Volume

127

Issue

5

Start / End Page

1332 / 1341

Location

United States

Related Subject Headings

  • Survival Rate
  • Respiratory System
  • RNA, Neoplasm
  • Oligonucleotide Array Sequence Analysis
  • Lymphatic Metastasis
  • Lymph Nodes
  • Lung Neoplasms
  • Humans
  • Genotype
  • Gene Expression Profiling