Evaluation of the blood stabilizers TransFix and Cyto-Chex BCT for low-cost CD4 T-cell methodologies.

Published

Journal Article

TransFix(TM) and Cyto-Chex((R)) BCT (blood collection tube) reagents have been shown to maintain whole blood integrity for delayed immunophenotyping by flow cytometry. We evaluated the ability of these blood-stabilizing reagents to preserve HIV-seropositive blood for delayed CD4(+) T-cell quantification utilizing the Dynal((R)) Biotech T4 Quant Kit. TransFix was added to EDTA-anticoagulated whole blood and tested at a 1:10 dilution over 7 d using the Dynal (n = 21) manual method. Compared to baseline analysis, a significant decrease in mean CD4(+) counts was observed over time. Cyto-Chex BCT-preserved samples (n = 20) were tested for CD4(+) counts by Dynal over 7 d, with storage at varying temperatures: room temperature (21 degrees C), 37 degrees C, and 37 degrees C with intermittent storage at 42 degrees C. A significant decline in mean CD4(+) counts was observed in samples at all temperatures compared to baseline (p < 0.05). Increases in temperature to and above 37 degrees C resulted in a greater decline in mean CD4(+) counts over time. Our findings indicated that neither TransFix or Cyto-Chex BCT was a suitable blood stabilizer when used for delayed CD4(+) quantification with a low-cost manual CD4(+) bead-based method.

Full Text

Duke Authors

Cited Authors

  • Plate, MM; Louzao, R; Steele, PM; Greengrass, V; Morris, LM; Lewis, J; Barnett, D; Warrino, D; Hearps, AC; Denny, T; Crowe, SM

Published Date

  • October 2009

Published In

Volume / Issue

  • 22 / 5

Start / End Page

  • 329 - 332

PubMed ID

  • 19811090

Pubmed Central ID

  • 19811090

Electronic International Standard Serial Number (EISSN)

  • 1557-8976

Digital Object Identifier (DOI)

  • 10.1089/vim.2009.0027

Language

  • eng

Conference Location

  • United States