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Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells.

Publication ,  Journal Article
de la Fuente, C; Wang, L; Wang, D; Deng, L; Wu, K; Li, H; Stein, LD; Denny, T; Coffman, F; Kehn, K; Baylor, S; Maddukuri, A; Pumfery, A; Kashanchi, F
Published in: Mol Cell Biochem
March 2003

Adult T-cell leukemia (ATL) and HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) are associated with Human T-cell lymphotropic virus type 1 (HTLV-1) infection. The viral transactivator, Tax is able to mediate the cell cycle progression by targeting key regulators of the cell cycle such as p21/waf1, p16/ink4a, p53, cyclins D1-3/cdk complexes, and the mitotic spindle checkpoint MAD apparatus, thereby deregulating cellular DNA damage and checkpoint control. Genome expression profiling of infected cells exemplified by the development of DNA microarrays represents a major advance in genome-wide functional analysis. Utilizing cDNA microarray analysis, we have observed an apparent opposing and paradoxical regulatory network of host cell gene expression upon the introduction of DNA damage stress signal. We find the apparent induction of cell cycle inhibitors, and pro- as well as anti-apoptotic gene expression is directly linked to whether cells are at either G1, S, or G2/M phases of the cell cycle. Specifically, a G1/S block is induced by p21/waf1 and p16/ink4a, while pro-apoptotic expression at S, and G2/M is associated with caspase activation, and anti-apoptotic gene expression is associated with up regulation of Bcl-2 family member, namely bfl-1 gene. Therefore, the microarray results indicating expression of both pro- and anti-apoptotic genes could easily be explained by the particular stage of the cell cycle. Mechanism and the functional outcome of induction for both pathways are discussed.

Duke Scholars

Published In

Mol Cell Biochem

DOI

ISSN

0300-8177

Publication Date

March 2003

Volume

245

Issue

1-2

Start / End Page

99 / 113

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Stress, Physiological
  • Signal Transduction
  • Mutation
  • Models, Biological
  • Mitosis
  • Leukemia-Lymphoma, Adult T-Cell
  • Humans
  • Human T-lymphotropic virus 1
 

Citation

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de la Fuente, C., Wang, L., Wang, D., Deng, L., Wu, K., Li, H., … Kashanchi, F. (2003). Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells. Mol Cell Biochem, 245(1–2), 99–113. https://doi.org/10.1023/a:1022866027585
Fuente, Cynthia de la, Lai Wang, Dai Wang, Longwen Deng, Kaili Wu, Hong Li, L Dana Stein, et al. “Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells.Mol Cell Biochem 245, no. 1–2 (March 2003): 99–113. https://doi.org/10.1023/a:1022866027585.
de la Fuente C, Wang L, Wang D, Deng L, Wu K, Li H, et al. Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells. Mol Cell Biochem. 2003 Mar;245(1–2):99–113.
de la Fuente, Cynthia, et al. “Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells.Mol Cell Biochem, vol. 245, no. 1–2, Mar. 2003, pp. 99–113. Pubmed, doi:10.1023/a:1022866027585.
de la Fuente C, Wang L, Wang D, Deng L, Wu K, Li H, Stein LD, Denny T, Coffman F, Kehn K, Baylor S, Maddukuri A, Pumfery A, Kashanchi F. Paradoxical effects of a stress signal on pro- and anti-apoptotic machinery in HTLV-1 Tax expressing cells. Mol Cell Biochem. 2003 Mar;245(1–2):99–113.
Journal cover image

Published In

Mol Cell Biochem

DOI

ISSN

0300-8177

Publication Date

March 2003

Volume

245

Issue

1-2

Start / End Page

99 / 113

Location

Netherlands

Related Subject Headings

  • Up-Regulation
  • Tumor Cells, Cultured
  • Stress, Physiological
  • Signal Transduction
  • Mutation
  • Models, Biological
  • Mitosis
  • Leukemia-Lymphoma, Adult T-Cell
  • Humans
  • Human T-lymphotropic virus 1