Enzyme immunoassay for detection of human immunodeficiency virus-specific immunoglobulin A antibodies.


Journal Article

Early diagnosis of human immunodeficiency virus (HIV) infection may be difficult in adults with acute or recent HIV infection and in infants with perinatally acquired HIV. Detection of HIV-specific immunoglobulin A (IgA) antibodies in infant serum by Western blot (immunoblot) has been suggested as a reliable method to identify HIV-infected infants, especially those over the age of 6 months, and as an adjunct to diagnosis of acute HIV infection in adults. We developed a simple enzyme immunoassay for detection of HIV-specific IgA, using standard commercially available reagents. Enzyme immunoassay was comparable to Western blot for detection of HIV-specific IgA in sera from adults (n = 216), older children (n = 49), and infants born to HIV-infected mothers (n = 65). Specificity was 100% and sensitivity ranged from 80 to 92%. IgA-enzyme immunoassay is a simple, highly sensitive method for detection of HIV-specific IgA antibodies and is easily adapted to the standard clinical laboratory.

Full Text

Duke Authors

Cited Authors

  • Connor, E; Wang, Z; Stephens, R; Holland, B; Palumbo, P; McSherry, G; Oleske, J; Denny, T

Published Date

  • March 1, 1993

Published In

Volume / Issue

  • 31 / 3

Start / End Page

  • 681 - 684

PubMed ID

  • 8458962

Pubmed Central ID

  • 8458962

International Standard Serial Number (ISSN)

  • 0095-1137


  • eng

Conference Location

  • United States