Characterization of B-cell type chronic lymphocytic leukemia cells by surface markers and a monoclonal antibody.
Journal Article (Journal Article)
This study was carried out to determine the reactivity of the Leu-1 mouse monoclonal antibody with B-chronic lymphocytic leukemia and other B-cell leukemias. This antibody has been previously reported to recognize a surface antigen expressed by almost all human thymocytes and peripheral T cells. It was also detected on surface immunoglobulin-bearing cells of most patients with chronic lymphocytic leukemia but was not detectable in normal B-cells and B-cell lines. In the present series, the neoplastic lymphocytes in 33 cases of B-chronic lymphocytic leukemia expressed surface immunoglobulin, Ia antigen, and receptors for Fc, C3, and mouse erythrocytes. All but one case expressed the Leu-1 antigen as detected by indirect immunofluorescence using flow cytometry. In three other cases, the leukemic cells in the peripheral blood reacted with anti-Leu-1, whereas the bone marrow lymphocytes did not. Moreover, quantitative differences in the surface density of Leu-1 were apparent by flow cytometry. The peripheral blood and bone marrow lymphocytes in other B-cell leukemias, including 15 cases of leukemic B-cell lymphomas and three cases of hairy-cell leukemia, failed to stain positively with the anti-Leu-1 antibody. The recognition of the Leu-1 antigen adds to the phenotypic characterization of b-chronic lymphocytic leukemia and may contribute to a better understanding of the pathogenesis of the disease and its expression in different tissues.
Full Text
Duke Authors
Cited Authors
- Koziner, B; Gebhard, D; Denny, T; Evans, RL
Published Date
- December 1, 1982
Published In
Volume / Issue
- 73 / 6
Start / End Page
- 802 - 807
PubMed ID
- 6756136
International Standard Serial Number (ISSN)
- 0002-9343
Digital Object Identifier (DOI)
- 10.1016/0002-9343(82)90761-6
Language
- eng
Conference Location
- United States