Skip to main content

Factors controlling oxygen utilization.

Publication ,  Journal Article
Biaglow, J; Dewhirst, M; Leeper, D; Burd, R; Tuttle, S
Published in: Adv Exp Med Biol
2005

We demonstrate, theoretically, that oxygen diffusion distance is related to the metabolic rate of tumors (QO2) as well as the oxygen tension. The difference in QO2 rate between tumors can vary by as much as 80-fold. Inhibition of oxygen utilization by glucose or chemical inhibitors can improve the diffusion distance. Combining respiratory inhibitors with increased availability of oxygen will further improve the oxygen diffusion distance for all tumors. A simple means for inhibiting oxygen consumption is the use of glucose (the Crabtree effect). The inhibition of tumor oxygen utilization by glucose occurs in R323OAc mammary carcinoma and 9L glioma cells. However, stimulation of oxygen consumption is observed with glucose in the Q7 hepatoma cell line. MIBG, a known inhibitor of oxygen utilization, blocks oxygen consumption in 9L, but is weakly inhibitory with the Q7. Q7 tumor cells demonstrate an anomalous behavior of glucose and MIBG on oxygen consumption. Our results clearly demonstrate the necessity for comparing effects of different agents on different tumor cells. Generalizations cannot be made with respect to the choice of inhibitor for in vivo use. Our work shows that oxygen consumption also can be inhibited with malonate and chlorosuccinate. These substrates may be effective in vivo, where glucose is low and glutamine is the major substrate. Our results indicate that information about individual tumor substrate-linked metabolic controls may be necessary before attempting to inhibit oxygen utilization in vivo for therapeutic benefit.

Duke Scholars

Published In

Adv Exp Med Biol

DOI

ISSN

0065-2598

Publication Date

2005

Volume

566

Start / End Page

317 / 323

Location

United States

Related Subject Headings

  • Succinic Acid
  • Rats
  • Oxygen Consumption
  • Neoplasms, Experimental
  • Mammary Neoplasms, Experimental
  • Malonates
  • Glutamine
  • Glucose
  • Glioma
  • General & Internal Medicine
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Biaglow, J., Dewhirst, M., Leeper, D., Burd, R., & Tuttle, S. (2005). Factors controlling oxygen utilization. Adv Exp Med Biol, 566, 317–323. https://doi.org/10.1007/0-387-26206-7_42
Biaglow, John, Mark Dewhirst, Dennis Leeper, Randy Burd, and Steve Tuttle. “Factors controlling oxygen utilization.Adv Exp Med Biol 566 (2005): 317–23. https://doi.org/10.1007/0-387-26206-7_42.
Biaglow J, Dewhirst M, Leeper D, Burd R, Tuttle S. Factors controlling oxygen utilization. Adv Exp Med Biol. 2005;566:317–23.
Biaglow, John, et al. “Factors controlling oxygen utilization.Adv Exp Med Biol, vol. 566, 2005, pp. 317–23. Pubmed, doi:10.1007/0-387-26206-7_42.
Biaglow J, Dewhirst M, Leeper D, Burd R, Tuttle S. Factors controlling oxygen utilization. Adv Exp Med Biol. 2005;566:317–323.

Published In

Adv Exp Med Biol

DOI

ISSN

0065-2598

Publication Date

2005

Volume

566

Start / End Page

317 / 323

Location

United States

Related Subject Headings

  • Succinic Acid
  • Rats
  • Oxygen Consumption
  • Neoplasms, Experimental
  • Mammary Neoplasms, Experimental
  • Malonates
  • Glutamine
  • Glucose
  • Glioma
  • General & Internal Medicine