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Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos.

Publication ,  Journal Article
Garner, LVT; Di Giulio, RT
Published in: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
May 2012

Glutathione transferases (GSTs) are phase II enzymes that detoxify a wide range of toxicants and reactive intermediates. One such class of toxicants is the ubiquitous polycyclic aromatic hydrocarbons (PAHs). Certain PAHs are known to cause developmental cardiac toxicity in fish. Herein, we explored the role of GST pi class 2 (GSTp2) in PAH- and PCB-induced cardiac toxicity in zebrafish (Danio rerio) embryos. We measured expression of GSTp2 in embryos exposed to individual and co-exposures of the PAHs benzo[k]fluoranthene (BkF), benzo[a]pyrene (BaP), and fluoranthene (FL) as well as 3,3',4,4',5-pentachlorobiphenyl (PCB-126). GSTp2 mRNA expression was induced by exposure to BkF, BaP, PCB-126, and BaP+FL and BkF+FL co-exposure. A splice junction morpholino was then used to knockdown GSTp2 in developing zebrafish. GSTp2 knockdown exacerbated the toxicity caused by co-exposures to BkF+FL and BaP+FL. However, GSTp2 knockdown did not affect PCB-126 toxicity. These results further suggest that pi class GSTs serve a protective function against the synergistic toxicity caused by PAHs in developing zebrafish.

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Published In

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

DOI

ISSN

1532-0456

Publication Date

May 2012

Volume

155

Issue

4

Start / End Page

573 / 579

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Polycyclic Aromatic Hydrocarbons
  • Polychlorinated Biphenyls
  • Morpholinos
  • Heart Defects, Congenital
  • Glutathione S-Transferase pi
  • Gene Silencing
  • Gene Knockdown Techniques
  • Gene Expression Regulation, Developmental
 

Citation

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Garner, L. V. T., & Di Giulio, R. T. (2012). Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 155(4), 573–579. https://doi.org/10.1016/j.cbpc.2012.01.007
Garner, Lindsey V. T., and Richard T. Di Giulio. “Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos.Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP 155, no. 4 (May 2012): 573–79. https://doi.org/10.1016/j.cbpc.2012.01.007.
Garner LVT, Di Giulio RT. Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. Comparative biochemistry and physiology Toxicology & pharmacology : CBP. 2012 May;155(4):573–9.
Garner, Lindsey V. T., and Richard T. Di Giulio. “Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos.Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 155, no. 4, May 2012, pp. 573–79. Epmc, doi:10.1016/j.cbpc.2012.01.007.
Garner LVT, Di Giulio RT. Glutathione transferase pi class 2 (GSTp2) protects against the cardiac deformities caused by exposure to PAHs but not PCB-126 in zebrafish embryos. Comparative biochemistry and physiology Toxicology & pharmacology : CBP. 2012 May;155(4):573–579.
Journal cover image

Published In

Comparative biochemistry and physiology. Toxicology & pharmacology : CBP

DOI

ISSN

1532-0456

Publication Date

May 2012

Volume

155

Issue

4

Start / End Page

573 / 579

Related Subject Headings

  • Zebrafish
  • Toxicology
  • Polycyclic Aromatic Hydrocarbons
  • Polychlorinated Biphenyls
  • Morpholinos
  • Heart Defects, Congenital
  • Glutathione S-Transferase pi
  • Gene Silencing
  • Gene Knockdown Techniques
  • Gene Expression Regulation, Developmental