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Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions.

Publication ,  Journal Article
Washburn, PC; Di Giulio, RT
Published in: Toxicology and applied pharmacology
September 1988

Nitroaromatic compounds, which frequently contaminate the environment, are known to be reduced to corresponding aromatic amines by fish as well as mammals under anaerobic conditions. Although amine products are not generally formed aerobically, "nitroreductase"-mediated redox cycling of nitroaromatics may occur under these conditions, leading to enhanced production of a potentially toxic oxygen species, superoxide (O-2). In this study, we have investigated the ability of channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions to stimulate O-2) production upon exposure to a model redox cycling nitroaromatic compound, nitrofurantoin (NF). Two assays for O-2 production, cytochrome c reduction and cyanide-insensitive oxygen consumption, were stimulated by NF exposure to both hepatic fractions. These reactions were partially inhibited by superoxide dismutase (SOD), and by SOD and catalase in the oxygen consumption assay, providing specific evidence for the involvement of O-2 in the stimulatory effect by NF. Furthermore, results of cofactor requirement and inhibition studies suggest that NF enhancement of O-2 production was mediated by NADPH-cytochrome P-450 (c) reductase in the microsomal fraction and xanthine oxidase in the soluble fraction. These findings comprehensively suggest that the in vitro stimulation of O-2 production by nitroaromatics as indicated in mammals may also occur in fish and, therefore, suggests a similar potential for oxyradical-mediated toxicities in these species.

Duke Scholars

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

September 1988

Volume

95

Issue

3

Start / End Page

363 / 377

Related Subject Headings

  • Toxicology
  • Superoxides
  • Superoxide Dismutase
  • Oxygen Consumption
  • Oxidation-Reduction
  • Nitrofurantoin
  • NADP
  • Microsomes, Liver
  • Liver
  • In Vitro Techniques
 

Citation

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ICMJE
MLA
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Washburn, P. C., & Di Giulio, R. T. (1988). Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions. Toxicology and Applied Pharmacology, 95(3), 363–377. https://doi.org/10.1016/0041-008x(88)90355-9
Washburn, P. C., and R. T. Di Giulio. “Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions.Toxicology and Applied Pharmacology 95, no. 3 (September 1988): 363–77. https://doi.org/10.1016/0041-008x(88)90355-9.
Washburn PC, Di Giulio RT. Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions. Toxicology and applied pharmacology. 1988 Sep;95(3):363–77.
Washburn, P. C., and R. T. Di Giulio. “Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions.Toxicology and Applied Pharmacology, vol. 95, no. 3, Sept. 1988, pp. 363–77. Epmc, doi:10.1016/0041-008x(88)90355-9.
Washburn PC, Di Giulio RT. Nitrofurantoin-stimulated superoxide production by channel catfish (Ictalurus punctatus) hepatic microsomal and soluble fractions. Toxicology and applied pharmacology. 1988 Sep;95(3):363–377.
Journal cover image

Published In

Toxicology and applied pharmacology

DOI

EISSN

1096-0333

ISSN

0041-008X

Publication Date

September 1988

Volume

95

Issue

3

Start / End Page

363 / 377

Related Subject Headings

  • Toxicology
  • Superoxides
  • Superoxide Dismutase
  • Oxygen Consumption
  • Oxidation-Reduction
  • Nitrofurantoin
  • NADP
  • Microsomes, Liver
  • Liver
  • In Vitro Techniques