Hematopoietic stem cells convert into liver cells within days without fusion.

Published

Journal Article

Both plasticity and cell fusion have been suggested to have a role in germ-layer switching. To understand the mechanisms underlying cell fate changes, we have examined a highly enriched population of hematopoietic stem cells (HSCs) in vitro or in vivo in response to injury for liver-specific phenotypic and functional changes. Here we show that HSCs become liver cells when cocultured with injured liver separated by a barrier. Chromosomal analyses and tissue-specific gene and/or protein expression show that microenvironmental cues rather than fusion are responsible for conversion in vitro. We transplanted HSCs into liver-injured mice and observed that HSCs convert into viable hepatocytes with increasing injury. Notably, liver function was restored 2-7 d after transplantation. We conclude that HSCs contribute to the regeneration of injured liver by converting into functional hepatocytes without fusion.

Full Text

Duke Authors

Cited Authors

  • Jang, Y-Y; Collector, MI; Baylin, SB; Diehl, AM; Sharkis, SJ

Published Date

  • June 2004

Published In

Volume / Issue

  • 6 / 6

Start / End Page

  • 532 - 539

PubMed ID

  • 15133469

Pubmed Central ID

  • 15133469

International Standard Serial Number (ISSN)

  • 1465-7392

Digital Object Identifier (DOI)

  • 10.1038/ncb1132

Language

  • eng

Conference Location

  • England