Altered cyclic AMP-dependent human chorionic gonadotropin production in cultured human placental trophoblasts exposed to ethanol.

Published

Journal Article

Chronic ethanol abuse during pregnancy can cause fetal injury, including the fetal alcohol syndrome (FAS). A contributing factor in this fetal injury may be the effect of ethanol on placental function. Previous studies have shown that ethanol treatment increases human chorionic gonadotropin (hCG) production by cultured human placental trophoblasts. In this study, we demonstrated that the stimulation of hCG production correlates with the ethanol concentration. Ethanol treatment enhanced intracellular adenosine 3':5'-cyclic monophosphate (cAMP) levels in response to either cholera toxin (CTX) or forskolin (FSK). Moreover, basal (i.e. unstimulated) cAMP levels were increased at 2 hr of ethanol exposure. However, this effect did not persist throughout the 24-hr incubation period. Therefore, ethanol treatment appears to induce increased hCG production, secondary to enhanced basal or stimulated cAMP production. The effect of ethanol was not associated with changes in Gs or Gi2 expression, as determined by northern blot and western blot analyses. In plasma membrane preparations from ethanol-treated cells, cAMP production was higher in response to Mn2+, a direct stimulator of adenylyl cyclase. Inclusion of Rp-cAMP, a protein kinase A inhibitor, eliminated the ethanol effect on hCG production. Treatment of cells with 8-Br-cAMP stimulated hCG production, but there was no difference between the ethanol-naive control and the ethanol-treated cells. These data suggest that ethanol treatment increases in vitro hCG production in human placental trophoblasts by enhancing cAMP production. Ethanol treatment appears to increase trophoblast adenylyl cyclase activity.

Full Text

Duke Authors

Cited Authors

  • Karl, PI; Divald, A; Diehl, AM; Fisher, SE

Published Date

  • January 1, 1998

Published In

Volume / Issue

  • 55 / 1

Start / End Page

  • 45 - 51

PubMed ID

  • 9413929

Pubmed Central ID

  • 9413929

International Standard Serial Number (ISSN)

  • 0006-2952

Digital Object Identifier (DOI)

  • 10.1016/s0006-2952(97)00404-8

Language

  • eng

Conference Location

  • England