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The role of local backrub motions in evolved and designed mutations.

Publication ,  Journal Article
Keedy, DA; Georgiev, I; Triplett, EB; Donald, BR; Richardson, DC; Richardson, JS
Published in: PLoS Comput Biol
2012

Amino acid substitutions in protein structures often require subtle backbone adjustments that are difficult to model in atomic detail. An improved ability to predict realistic backbone changes in response to engineered mutations would be of great utility for the blossoming field of rational protein design. One model that has recently grown in acceptance is the backrub motion, a low-energy dipeptide rotation with single-peptide counter-rotations, that is coupled to dynamic two-state sidechain rotamer jumps, as evidenced by alternate conformations in very high-resolution crystal structures. It has been speculated that backrubs may facilitate sequence changes equally well as rotamer changes. However, backrub-induced shifts and experimental uncertainty are of similar magnitude for backbone atoms in even high-resolution structures, so comparison of wildtype-vs.-mutant crystal structure pairs is not sufficient to directly link backrubs to mutations. In this study, we use two alternative approaches that bypass this limitation. First, we use a quality-filtered structure database to aggregate many examples for precisely defined motifs with single amino acid differences, and find that the effectively amplified backbone differences closely resemble backrubs. Second, we directly apply a provably-accurate, backrub-enabled protein design algorithm to idealized versions of these motifs, and discover that the lowest-energy computed models match the average-coordinate experimental structures. These results support the hypothesis that backrubs participate in natural protein evolution and validate their continued use for design of synthetic proteins.

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Published In

PLoS Comput Biol

DOI

EISSN

1553-7358

Publication Date

2012

Volume

8

Issue

8

Start / End Page

e1002629

Location

United States

Related Subject Headings

  • Uncertainty
  • Mutation
  • Movement
  • Bioinformatics
  • Amino Acids
  • Algorithms
  • 08 Information and Computing Sciences
  • 06 Biological Sciences
  • 01 Mathematical Sciences
 

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Keedy, D. A., Georgiev, I., Triplett, E. B., Donald, B. R., Richardson, D. C., & Richardson, J. S. (2012). The role of local backrub motions in evolved and designed mutations. PLoS Comput Biol, 8(8), e1002629. https://doi.org/10.1371/journal.pcbi.1002629
Keedy, Daniel A., Ivelin Georgiev, Edward B. Triplett, Bruce R. Donald, David C. Richardson, and Jane S. Richardson. “The role of local backrub motions in evolved and designed mutations.PLoS Comput Biol 8, no. 8 (2012): e1002629. https://doi.org/10.1371/journal.pcbi.1002629.
Keedy DA, Georgiev I, Triplett EB, Donald BR, Richardson DC, Richardson JS. The role of local backrub motions in evolved and designed mutations. PLoS Comput Biol. 2012;8(8):e1002629.
Keedy, Daniel A., et al. “The role of local backrub motions in evolved and designed mutations.PLoS Comput Biol, vol. 8, no. 8, 2012, p. e1002629. Pubmed, doi:10.1371/journal.pcbi.1002629.
Keedy DA, Georgiev I, Triplett EB, Donald BR, Richardson DC, Richardson JS. The role of local backrub motions in evolved and designed mutations. PLoS Comput Biol. 2012;8(8):e1002629.

Published In

PLoS Comput Biol

DOI

EISSN

1553-7358

Publication Date

2012

Volume

8

Issue

8

Start / End Page

e1002629

Location

United States

Related Subject Headings

  • Uncertainty
  • Mutation
  • Movement
  • Bioinformatics
  • Amino Acids
  • Algorithms
  • 08 Information and Computing Sciences
  • 06 Biological Sciences
  • 01 Mathematical Sciences