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Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation.

Publication ,  Journal Article
Cittadini, A; Strömer, H; Katz, SE; Clark, R; Moses, AC; Morgan, JP; Douglas, PS
Published in: Circulation
February 15, 1996

BACKGROUND: Despite their increasing clinical use and recent evidence that growth hormone (GH) and insulin-like growth factor-1 (IGF-1) target the heart, there has been no systematic investigation of the effects of GH and IGF-1 on the cardiovascular system. METHODS AND RESULTS: Sixty normal but growing adult female rats were randomized to receive 4 weeks of treatment with GH (3.5 mg.kg-1.d-1), IGF-1 (3 mg.kg-1.d-1), a combination of the two, or placebo. Transthoracic echocardiograms were performed at baseline and at 2 weeks and 4 weeks of treatment. After the final echocardiography, rats underwent either closed-chest left ventricular (LV) catheterization or Langendorff perfusion studies. Myocyte diameter and interstitial tissue fraction were assessed by morphometric histology. Echocardiographic and ex vivo data demonstrated a LV hypertrophic response in all three groups of treated animals that was most marked in the GH group, which alone exhibited a concentric growth pattern (relative wall thickness, 0.52 versus 0.42 to 0.44 in the other groups; P < .001). At 4 weeks, cardiac index was significantly higher and total systemic vascular resistance was lower in all groups of treated animals than in control animals (both P < .001), whereas arterial blood pressure did not differ significantly. All indexes of in vivo and in vitro cardiac function were higher in GH- and IGF-1-treated rats than in control animals, whereas combination therapy yielded a blunted effect. Myocyte diameter was increased in all three treated groups without an increase in interstitial tissue. CONCLUSIONS: Exogenous administration of GH and IGF-1 in the normal adult rat induces a cardiac hypertrophic response without development of significant fibrosis. Cardiac performance is increased both in vivo and in the isolated heart.

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Published In

Circulation

DOI

ISSN

0009-7322

Publication Date

February 15, 1996

Volume

93

Issue

4

Start / End Page

800 / 809

Location

United States

Related Subject Headings

  • Ventricular Function, Left
  • Rats, Sprague-Dawley
  • Rats
  • Myocardial Contraction
  • Insulin-Like Growth Factor I
  • In Vitro Techniques
  • Hypertrophy, Left Ventricular
  • Humans
  • Hemodynamics
  • Heart
 

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Cittadini, A., Strömer, H., Katz, S. E., Clark, R., Moses, A. C., Morgan, J. P., & Douglas, P. S. (1996). Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation. Circulation, 93(4), 800–809. https://doi.org/10.1161/01.cir.93.4.800
Cittadini, A., H. Strömer, S. E. Katz, R. Clark, A. C. Moses, J. P. Morgan, and P. S. Douglas. “Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation.Circulation 93, no. 4 (February 15, 1996): 800–809. https://doi.org/10.1161/01.cir.93.4.800.
Cittadini A, Strömer H, Katz SE, Clark R, Moses AC, Morgan JP, et al. Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation. Circulation. 1996 Feb 15;93(4):800–9.
Cittadini, A., et al. “Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation.Circulation, vol. 93, no. 4, Feb. 1996, pp. 800–09. Pubmed, doi:10.1161/01.cir.93.4.800.
Cittadini A, Strömer H, Katz SE, Clark R, Moses AC, Morgan JP, Douglas PS. Differential cardiac effects of growth hormone and insulin-like growth factor-1 in the rat. A combined in vivo and in vitro evaluation. Circulation. 1996 Feb 15;93(4):800–809.

Published In

Circulation

DOI

ISSN

0009-7322

Publication Date

February 15, 1996

Volume

93

Issue

4

Start / End Page

800 / 809

Location

United States

Related Subject Headings

  • Ventricular Function, Left
  • Rats, Sprague-Dawley
  • Rats
  • Myocardial Contraction
  • Insulin-Like Growth Factor I
  • In Vitro Techniques
  • Hypertrophy, Left Ventricular
  • Humans
  • Hemodynamics
  • Heart