Assessing incomplete deprotection of microarray oligonucleotides in situ.

Journal Article

En masse analysis of gene structure and function by array technologies will have a lasting and profound effect on biology and medicine. This impact can be compromised by low quality of probes within arrays, which we show can be caused by incomplete removal of chemical protecting groups. To solve this quality control problem, we present a sensitive, specific and facile method to detect these groups in situ on arrays using monoclonal antibodies and existing instrumentation. Screening of microarrays with these monoclonal antibodies should guide the consideration given to data derived from these and should enhance the accuracy of the results obtained.

Full Text

Duke Authors

Cited Authors

  • Dressman, HK; Barley-Maloney, L; Rowlette, L-L; Agris, PF; Garcia-Blanco, MA

Published Date

  • 2006

Published In

Volume / Issue

  • 34 / 19

Start / End Page

  • e131 -

PubMed ID

  • 17020916

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Digital Object Identifier (DOI)

  • 10.1093/nar/gkl713

Language

  • eng

Conference Location

  • England