Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.

Published

Journal Article

The Tg.AC mouse carrying the v-Ha-ras structural gene is a useful model for the study of chemical carcinogens, especially those acting via non-genotoxic mechanisms. This study evaluated the efficacy of the non-toxic, water-soluble antioxidant from spinach, natural antioxidant (NAO), in reducing skin papilloma induction in female hemizygous Tg.AC mice treated dermally five times over 2.5 weeks with 2.5 microg 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-only group was considered as a control; the other two groups received, additionally, NAO topically (2 mg) or orally (100 mg/kg), 5 days/week for 5 weeks. Papilloma counts made macroscopically during the clinical observations showed a significant decrease in multiplicity (P<0.01) in the NAO topically treated group. According to histological criteria, papilloma multiplicity were lower in both topical-NAO and oral-NAO groups, but significantly so only in the oral-NAO mice (P<0.01). The beneficial effect of NAO in the Tg.AC mouse is reported.

Full Text

Duke Authors

Cited Authors

  • Nyska, A; Lomnitski, L; Spalding, J; Dunson, DB; Goldsworthy, TL; Ben-Shaul, V; Grossman, S; Bergman, M; Boorman, G

Published Date

  • May 2001

Published In

Volume / Issue

  • 122 / 1

Start / End Page

  • 33 - 44

PubMed ID

  • 11397555

Pubmed Central ID

  • 11397555

Electronic International Standard Serial Number (EISSN)

  • 1879-3169

International Standard Serial Number (ISSN)

  • 0378-4274

Digital Object Identifier (DOI)

  • 10.1016/s0378-4274(01)00345-9

Language

  • eng