Scholars@Duke publication: Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.

Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.

Publication , Journal Article

Nyska, A; Lomnitski, L; Spalding, J; Dunson, DB; Goldsworthy, TL; Ben-Shaul, V; Grossman, S; Bergman, M; Boorman, G

Published in: Toxicology letters

May 2001

The Tg.AC mouse carrying the v-Ha-ras structural gene is a useful model for the study of chemical carcinogens, especially those acting via non-genotoxic mechanisms. This study evaluated the efficacy of the non-toxic, water-soluble antioxidant from spinach, natural antioxidant (NAO), in reducing skin papilloma induction in female hemizygous Tg.AC mice treated dermally five times over 2.5 weeks with 2.5 microg 12-O-tetradecanoylphorbol-13-acetate (TPA). The TPA-only group was considered as a control; the other two groups received, additionally, NAO topically (2 mg) or orally (100 mg/kg), 5 days/week for 5 weeks. Papilloma counts made macroscopically during the clinical observations showed a significant decrease in multiplicity (P<0.01) in the NAO topically treated group. According to histological criteria, papilloma multiplicity were lower in both topical-NAO and oral-NAO groups, but significantly so only in the oral-NAO mice (P<0.01). The beneficial effect of NAO in the Tg.AC mouse is reported.

Duke Scholars

Author David B. Dunson Statistical Science

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Published In

Toxicology letters

DOI

10.1016/s0378-4274(01)00345-9

EISSN

1879-3169

ISSN

0378-4274

Publication Date

May 2001

Volume

122

Issue

Start / End Page

33 / 44

Related Subject Headings

Toxicology
Tetradecanoylphorbol Acetate
Survival Analysis
Spinacia oleracea
Skin Neoplasms
Plant Extracts
Papilloma
Mice, Transgenic
Mice
Genotype

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Nyska, A., Lomnitski, L., Spalding, J., Dunson, D. B., Goldsworthy, T. L., Ben-Shaul, V., … Boorman, G. (2001). Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model. Toxicology Letters, 122(1), 33–44. https://doi.org/10.1016/s0378-4274(01)00345-9

Nyska, A., L. Lomnitski, J. Spalding, D. B. Dunson, T. L. Goldsworthy, V. Ben-Shaul, S. Grossman, M. Bergman, and G. Boorman. “Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.” Toxicology Letters 122, no. 1 (May 2001): 33–44. https://doi.org/10.1016/s0378-4274(01)00345-9.

Nyska A, Lomnitski L, Spalding J, Dunson DB, Goldsworthy TL, Ben-Shaul V, et al. Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model. Toxicology letters. 2001 May;122(1):33–44.

Nyska, A., et al. “Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model.” Toxicology Letters, vol. 122, no. 1, May 2001, pp. 33–44. Epmc, doi:10.1016/s0378-4274(01)00345-9.

Nyska A, Lomnitski L, Spalding J, Dunson DB, Goldsworthy TL, Ben-Shaul V, Grossman S, Bergman M, Boorman G. Topical and oral administration of the natural water-soluble antioxidant from spinach reduces the multiplicity of papillomas in the Tg.AC mouse model. Toxicology letters. 2001 May;122(1):33–44.