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Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium.

Publication ,  Journal Article
Wu, Y; Ip, JE; Huang, J; Zhang, L; Matsushita, K; Liew, C-C; Pratt, RE; Dzau, VJ
Published in: Circ Res
August 4, 2006

Bone marrow-derived endothelial progenitor cells (EPCs) have the ability to migrate to ischemic organs. However, the signals that mediate trafficking and recruitment of these cells are not well understood. Using a functional genomics strategy, we determined the genes that were upregulated in the ischemic myocardium and might be involved in EPC recruitment. Among them, CD18 and its ligand ICAM-1 are particularly intriguing because CD18 and its heterodimer binding chains CD11a and CD11b were correspondingly expressed in ex vivo-expanded EPCs isolated from rat and murine bone marrows. To further verify the functional role of CD18 in mediating EPC recruitment and repair to the infarcted myocardium, we used neutralizing antibody to block CD18. Blockade of CD18 in EPCs significantly inhibited their attachment capacity in vitro and reduced their recruitment to the ischemic myocardium in vivo by 95%. Moreover, mice receiving EPCs that were treated with control isotype IgG exhibited significantly increased capillary density in the infarct border zone, reduced cardiac dilatation, ventricular wall thinning, and fibrosis when compared with myocardial infarction mice receiving PBS and CD18 blockade reversed the EPC-mediated improvements to the infarcted heart. Thus, our results suggest an essential role of CD18 in mediating EPC recruitment and the subsequent functional effects on the infarcted heart.

Duke Scholars

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Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

August 4, 2006

Volume

99

Issue

3

Start / End Page

315 / 322

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transfection
  • Stem Cells
  • Stem Cell Transplantation
  • Regeneration
  • Rats, Sprague-Dawley
  • Rats
  • Neovascularization, Physiologic
  • Myocardial Ischemia
  • Myocardial Infarction
 

Citation

APA
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MLA
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Wu, Y., Ip, J. E., Huang, J., Zhang, L., Matsushita, K., Liew, C.-C., … Dzau, V. J. (2006). Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium. Circ Res, 99(3), 315–322. https://doi.org/10.1161/01.RES.0000235986.35957.a3
Wu, Yaojiong, James E. Ip, Jing Huang, Lunan Zhang, Kenichi Matsushita, Choong-Chin Liew, Richard E. Pratt, and Victor J. Dzau. “Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium.Circ Res 99, no. 3 (August 4, 2006): 315–22. https://doi.org/10.1161/01.RES.0000235986.35957.a3.
Wu Y, Ip JE, Huang J, Zhang L, Matsushita K, Liew C-C, et al. Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium. Circ Res. 2006 Aug 4;99(3):315–22.
Wu, Yaojiong, et al. “Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium.Circ Res, vol. 99, no. 3, Aug. 2006, pp. 315–22. Pubmed, doi:10.1161/01.RES.0000235986.35957.a3.
Wu Y, Ip JE, Huang J, Zhang L, Matsushita K, Liew C-C, Pratt RE, Dzau VJ. Essential role of ICAM-1/CD18 in mediating EPC recruitment, angiogenesis, and repair to the infarcted myocardium. Circ Res. 2006 Aug 4;99(3):315–322.

Published In

Circ Res

DOI

EISSN

1524-4571

Publication Date

August 4, 2006

Volume

99

Issue

3

Start / End Page

315 / 322

Location

United States

Related Subject Headings

  • Up-Regulation
  • Transfection
  • Stem Cells
  • Stem Cell Transplantation
  • Regeneration
  • Rats, Sprague-Dawley
  • Rats
  • Neovascularization, Physiologic
  • Myocardial Ischemia
  • Myocardial Infarction