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Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy.

Publication ,  Journal Article
Griese, DP; Ehsan, A; Melo, LG; Kong, D; Zhang, L; Mann, MJ; Pratt, RE; Mulligan, RC; Dzau, VJ
Published in: Circulation
November 25, 2003

BACKGROUND: Blood-borne endothelial cells originating from adult bone marrow were reported previously. These cells have the properties of an endothelial progenitor cell (EPC) and can be mobilized by cytokines and recruited to sites of neovascularization, where they differentiate into mature endothelial cells. Current protocols for isolation of EPCs from peripheral blood rely on enrichment and selection of CD34+ mononuclear cells. METHODS AND RESULTS: In this report, we describe a streamlined method for the isolation and expansion of EPCs from peripheral blood and evaluate their therapeutic potential for autologous cell-based therapy of injured blood vessels and prosthetic grafts. A subset of unfractionated mononuclear cells exhibited the potential to differentiate in vitro into endothelial cells under selective growth conditions. The cells were efficiently transduced ex vivo by a retroviral vector expressing the LacZ reporter gene and could be expanded to yield sufficient numbers for therapeutic applications. Transplantation of these cells into balloon-injured carotid arteries and into bioprosthetic grafts in rabbits led to rapid endothelialization of the denuded vessels and graft segments, resulting in significant reduction in neointima deposition. CONCLUSIONS: We conclude that transplantation of EPCs may play a crucial role in reestablishing endothelial integrity in injured vessels, thereby inhibiting neointimal hyperplasia. These findings may have implications for novel and practical cell-based therapies for vascular disease.

Duke Scholars

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Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

November 25, 2003

Volume

108

Issue

21

Start / End Page

2710 / 2715

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Tunica Intima
  • Transplantation, Autologous
  • Retroviridae
  • Rabbits
  • Male
  • Leukocytes, Mononuclear
  • Hyperplasia
  • Graft Survival
  • Graft Occlusion, Vascular
 

Citation

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Griese, D. P., Ehsan, A., Melo, L. G., Kong, D., Zhang, L., Mann, M. J., … Dzau, V. J. (2003). Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. Circulation, 108(21), 2710–2715. https://doi.org/10.1161/01.CIR.0000096490.16596.A6
Griese, Daniel P., Afshin Ehsan, Luis G. Melo, Deling Kong, Lunan Zhang, Michael J. Mann, Richard E. Pratt, Richard C. Mulligan, and Victor J. Dzau. “Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy.Circulation 108, no. 21 (November 25, 2003): 2710–15. https://doi.org/10.1161/01.CIR.0000096490.16596.A6.
Griese, Daniel P., et al. “Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy.Circulation, vol. 108, no. 21, Nov. 2003, pp. 2710–15. Pubmed, doi:10.1161/01.CIR.0000096490.16596.A6.
Griese DP, Ehsan A, Melo LG, Kong D, Zhang L, Mann MJ, Pratt RE, Mulligan RC, Dzau VJ. Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prosthetic grafts: implications for cell-based vascular therapy. Circulation. 2003 Nov 25;108(21):2710–2715.

Published In

Circulation

DOI

EISSN

1524-4539

Publication Date

November 25, 2003

Volume

108

Issue

21

Start / End Page

2710 / 2715

Location

United States

Related Subject Headings

  • beta-Galactosidase
  • Tunica Intima
  • Transplantation, Autologous
  • Retroviridae
  • Rabbits
  • Male
  • Leukocytes, Mononuclear
  • Hyperplasia
  • Graft Survival
  • Graft Occlusion, Vascular