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Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells.

Publication ,  Journal Article
Tamura, K; Chen, YE; Lopez-Ilasaca, M; Daviet, L; Tamura, N; Ishigami, T; Akishita, M; Takasaki, I; Tokita, Y; Pratt, RE; Horiuchi, M ...
Published in: J Biol Chem
November 3, 2000

Fibronectin plays an important role in vascular remodeling. A functional interaction between mechanical stimuli and locally produced vasoactive agents is suggested to be crucial for vascular remodeling. We examined the effect of mechanical stretch on fibronectin gene expression in vascular smooth muscle cells and the role of vascular angiotensin II in the regulation of the fibronectin gene in response to stretch. Cyclic stretch induced an increase in vascular fibronectin mRNA levels that was inhibited by actinomycin D and CV11974, an angiotensin II type 1 receptor antagonist; cycloheximide and PD123319, an angiotensin II type 2 receptor antagonist, did not affect the induction. In transfection experiments, fibronectin promoter activity was stimulated by stretch and inhibited by CV11974 but not by PD123319. DNA-protein binding experiments revealed that cyclic stretch enhanced nuclear binding to the AP-1 site, which was partially supershifted by antibody to c-Jun. Site-directed mutation of the AP-1 site significantly decreased the cyclic stretch-mediated activation of fibronectin promoter. Furthermore, antisense c-jun oligonucleotides decreased the stretch-induced stimulation of the fibronectin promoter activity and the mRNA expression. These results suggest that cyclic stretch stimulates vascular fibronectin gene expression mainly via the activation of AP-1 through the angiotensin II type 1 receptor.

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Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

November 3, 2000

Volume

275

Issue

44

Start / End Page

34619 / 34627

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factor AP-1
  • Renin-Angiotensin System
  • Receptors, Angiotensin
  • RNA, Messenger
  • Protein Synthesis Inhibitors
  • Promoter Regions, Genetic
  • Muscle, Smooth, Vascular
  • Gene Expression Regulation
 

Citation

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Tamura, K., Chen, Y. E., Lopez-Ilasaca, M., Daviet, L., Tamura, N., Ishigami, T., … Umemura, S. (2000). Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells. J Biol Chem, 275(44), 34619–34627. https://doi.org/10.1074/jbc.M004421200
Tamura, K., Y. E. Chen, M. Lopez-Ilasaca, L. Daviet, N. Tamura, T. Ishigami, M. Akishita, et al. “Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells.J Biol Chem 275, no. 44 (November 3, 2000): 34619–27. https://doi.org/10.1074/jbc.M004421200.
Tamura K, Chen YE, Lopez-Ilasaca M, Daviet L, Tamura N, Ishigami T, et al. Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells. J Biol Chem. 2000 Nov 3;275(44):34619–27.
Tamura, K., et al. “Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells.J Biol Chem, vol. 275, no. 44, Nov. 2000, pp. 34619–27. Pubmed, doi:10.1074/jbc.M004421200.
Tamura K, Chen YE, Lopez-Ilasaca M, Daviet L, Tamura N, Ishigami T, Akishita M, Takasaki I, Tokita Y, Pratt RE, Horiuchi M, Dzau VJ, Umemura S. Molecular mechanism of fibronectin gene activation by cyclic stretch in vascular smooth muscle cells. J Biol Chem. 2000 Nov 3;275(44):34619–34627.

Published In

J Biol Chem

DOI

ISSN

0021-9258

Publication Date

November 3, 2000

Volume

275

Issue

44

Start / End Page

34619 / 34627

Location

United States

Related Subject Headings

  • Transcriptional Activation
  • Transcription, Genetic
  • Transcription Factor AP-1
  • Renin-Angiotensin System
  • Receptors, Angiotensin
  • RNA, Messenger
  • Protein Synthesis Inhibitors
  • Promoter Regions, Genetic
  • Muscle, Smooth, Vascular
  • Gene Expression Regulation