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Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy.

Publication ,  Journal Article
Morishita, R; Gibbons, GH; Kaneda, Y; Ogihara, T; Dzau, VJ
Published in: Biochem Biophys Res Commun
July 5, 2000

In this study, we examined the feasibility of a systemic administration of HVJ-liposome complex containing human insulin construct into the blood in mice via the tail vein. Transfection of human insulin vector resulted in a transient decrease in serum glucose in streptozotocin (SZT)-induced diabetic mice, accompanied by the detection of human insulin in the liver and spleen. In accordance with the decreased glucose, plasma immunoreactive insulin could be detected up to 14 days after a single transfection in mice transfected with insulin vector. Repeated intravenous injection of human insulin vector every week resulted in a sustained decrease in serum glucose over a 4-week period, accompanied by the detection of C-peptide fragments and a significant decrease in BUN and creatinine. Here, we demonstrated the feasibility of intravenous systemic administration of an insulin vector that results in a sustained improvement of diabetic glucose metabolism.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

July 5, 2000

Volume

273

Issue

2

Start / End Page

666 / 674

Location

United States

Related Subject Headings

  • Transfection
  • Respirovirus
  • Mice, Transgenic
  • Mice
  • Male
  • Liposomes
  • Insulin
  • Injections, Intravenous
  • Humans
  • Genetic Vectors
 

Citation

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Morishita, R., Gibbons, G. H., Kaneda, Y., Ogihara, T., & Dzau, V. J. (2000). Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy. Biochem Biophys Res Commun, 273(2), 666–674. https://doi.org/10.1006/bbrc.2000.2936
Morishita, R., G. H. Gibbons, Y. Kaneda, T. Ogihara, and V. J. Dzau. “Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy.Biochem Biophys Res Commun 273, no. 2 (July 5, 2000): 666–74. https://doi.org/10.1006/bbrc.2000.2936.
Morishita R, Gibbons GH, Kaneda Y, Ogihara T, Dzau VJ. Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy. Biochem Biophys Res Commun. 2000 Jul 5;273(2):666–74.
Morishita, R., et al. “Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy.Biochem Biophys Res Commun, vol. 273, no. 2, July 2000, pp. 666–74. Pubmed, doi:10.1006/bbrc.2000.2936.
Morishita R, Gibbons GH, Kaneda Y, Ogihara T, Dzau VJ. Systemic administration of HVJ viral coat-liposome complex containing human insulin vector decreases glucose level in diabetic mouse: A model of gene therapy. Biochem Biophys Res Commun. 2000 Jul 5;273(2):666–674.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

July 5, 2000

Volume

273

Issue

2

Start / End Page

666 / 674

Location

United States

Related Subject Headings

  • Transfection
  • Respirovirus
  • Mice, Transgenic
  • Mice
  • Male
  • Liposomes
  • Insulin
  • Injections, Intravenous
  • Humans
  • Genetic Vectors