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Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway.

Publication ,  Journal Article
Takeuchi, K; Nakamura, N; Cook, NS; Pratt, RE; Dzau, VJ
Published in: Biochem Biophys Res Commun
November 15, 1990

An expression vector containing three copies of the AP-1 binding element (TRE) upstream of a thymidine kinase promotor which controlled the expression of the chloramphenicol acetyl transferase (CAT) gene was transiently transfected into vascular smooth muscle (VSM) cells and a human hepatocarcinoma cell line, Hep G2. Twelve hours of angiotensin (Ang) II exposure stimulated significantly CAT expression by 3.4 fold and 2.7 fold in Hep G2 and VSM cells, respectively. AngII had no effect on CAT expression of a control vector. This AngII-induced stimulation was attenuated significantly by an AngII receptor antagonist, Sar1 Ile8 AngII, and abolished completely by a PKC inhibitor, staurosporine. Our data suggest that the TRE plays a crucial role in AngII-induced gene expression that is mediated by PKC. We concluded that TRE is one of the AngII-responsive elements.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 15, 1990

Volume

172

Issue

3

Start / End Page

1189 / 1194

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Thymidine Kinase
  • Staurosporine
  • Repetitive Sequences, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Angiotensin
  • Rats, Inbred Strains
  • Rats
  • Protein Kinase C
 

Citation

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Takeuchi, K., Nakamura, N., Cook, N. S., Pratt, R. E., & Dzau, V. J. (1990). Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway. Biochem Biophys Res Commun, 172(3), 1189–1194. https://doi.org/10.1016/0006-291x(90)91574-c
Takeuchi, K., N. Nakamura, N. S. Cook, R. E. Pratt, and V. J. Dzau. “Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway.Biochem Biophys Res Commun 172, no. 3 (November 15, 1990): 1189–94. https://doi.org/10.1016/0006-291x(90)91574-c.
Takeuchi K, Nakamura N, Cook NS, Pratt RE, Dzau VJ. Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway. Biochem Biophys Res Commun. 1990 Nov 15;172(3):1189–94.
Takeuchi, K., et al. “Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway.Biochem Biophys Res Commun, vol. 172, no. 3, Nov. 1990, pp. 1189–94. Pubmed, doi:10.1016/0006-291x(90)91574-c.
Takeuchi K, Nakamura N, Cook NS, Pratt RE, Dzau VJ. Angiotensin II can regulate gene expression by the AP-1 binding sequence via a protein kinase C-dependent pathway. Biochem Biophys Res Commun. 1990 Nov 15;172(3):1189–1194.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

November 15, 1990

Volume

172

Issue

3

Start / End Page

1189 / 1194

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transfection
  • Thymidine Kinase
  • Staurosporine
  • Repetitive Sequences, Nucleic Acid
  • Regulatory Sequences, Nucleic Acid
  • Receptors, Angiotensin
  • Rats, Inbred Strains
  • Rats
  • Protein Kinase C