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Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct.

Publication ,  Journal Article
Sigmund, CD; Okuyama, K; Ingelfinger, J; Jones, CA; Mullins, JJ; Kane, C; Kim, U; Wu, CZ; Kenny, L; Rustum, Y
Published in: J Biol Chem
November 15, 1990

We constructed transgenic mice containing a renin-promoter SV40 T antigen fusion transgene with the intention of inducing neoplasia in renin-expressing cells and isolating renin-expressing cell lines in vitro. We examined six kidney tumors from mice representing three different transgenic lines and found they expressed their endogenous renin gene. Initially, five nonclonal kidney tumor-derived cell lines were established which expressed their endogenous renin gene in addition to the transgene. They retained active renin intracellularly and constitutively secreted an inactive form of renin (prorenin). One of these cell lines was cloned to homogeneity. This line maintained high level expression of renin mRNA throughout 3 months of continuous culture. Although the cells contained an equal proportion of active and inactive renin, the species constitutively secreted into the media was predominantly (95%) prorenin. However, active renin secretion was stimulated 2.3- and 4.6-fold by treatment with 8-bromo-cAMP after 4 and 15 h, respectively. In addition, the presence of multiple secretory granules was confirmed by ultrastructural analysis. These cells, which express renin mRNA and can regulate secretion of active renin, should provide an excellent tool for studying renin gene regulation and secretion. Furthermore, these mice should provide a useful source for the establishment of renin-expressing cell lines from a variety of renin-expressing tissues.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 15, 1990

Volume

265

Issue

32

Start / End Page

19916 / 19922

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Simian virus 40
  • Renin
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Microscopy, Electron
  • Mice, Transgenic
  • Mice, Nude
  • Mice
  • Kidney Neoplasms
 

Citation

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Sigmund, C. D., Okuyama, K., Ingelfinger, J., Jones, C. A., Mullins, J. J., Kane, C., … Rustum, Y. (1990). Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J Biol Chem, 265(32), 19916–19922.
Sigmund, C. D., K. Okuyama, J. Ingelfinger, C. A. Jones, J. J. Mullins, C. Kane, U. Kim, C. Z. Wu, L. Kenny, and Y. Rustum. “Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct.J Biol Chem 265, no. 32 (November 15, 1990): 19916–22.
Sigmund CD, Okuyama K, Ingelfinger J, Jones CA, Mullins JJ, Kane C, et al. Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J Biol Chem. 1990 Nov 15;265(32):19916–22.
Sigmund CD, Okuyama K, Ingelfinger J, Jones CA, Mullins JJ, Kane C, Kim U, Wu CZ, Kenny L, Rustum Y. Isolation and characterization of renin-expressing cell lines from transgenic mice containing a renin-promoter viral oncogene fusion construct. J Biol Chem. 1990 Nov 15;265(32):19916–19922.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

November 15, 1990

Volume

265

Issue

32

Start / End Page

19916 / 19922

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Simian virus 40
  • Renin
  • RNA, Messenger
  • Promoter Regions, Genetic
  • Microscopy, Electron
  • Mice, Transgenic
  • Mice, Nude
  • Mice
  • Kidney Neoplasms