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Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion.

Publication ,  Journal Article
Tang, SS; Stevenson, L; Dzau, VJ
Published in: Circ Res
January 1990

Cultured bovine aortic endothelial cells (BAECs) express the complete renin-angiotensin system and secrete angiotensins. In this study, we examined the adrenergic influence on the secretion of angiotensins from BAECs. Angiotensins were determined by high-performance liquid chromatography and radioimmunoassay. At basal state, BAECs contain angiotensin I, angiotensin II, and angiotensin III at concentrations of 2.5 +/- 1.3, 4.8 +/- 2.3, and 3.4 +/- 1.5 pg/10(6) cells, respectively. Angiotensin I, angiotensin II, and angiotensin III concentrations in the culture medium were 8.3 +/- 4.4, 9.4 +/- 3.5, and 9.9 +/- 3.3 pg/10(6) cells, respectively. Isoproterenol (0.1-10 microM) increases secretion of angiotensins I, II, and III in a dose-dependent manner. Increase in angiotensin secretion induced by isoproterenol (10 microM) can be inhibited by beta 2-adrenoceptor antagonist ICI 118,551 (1 microM), but not by beta 1-adrenoceptor antagonist atenolol (1 microM). Forskolin (1-1,000 microM) mimics the isoproterenol-induced response. In contrast, alpha-adrenergic agonist phenylephrine (1-100 microM) inhibits the secretion. Pretreatment of BAECs with captopril (1 microM) inhibits the accumulation of angiotensin II and angiotensin III in the culture medium, but not angiotensin I. These findings suggest that BAEC production and/or secretion of angiotensins is regulated by adrenergic mechanisms.

Duke Scholars

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

January 1990

Volume

66

Issue

1

Start / End Page

103 / 108

Location

United States

Related Subject Headings

  • Renin-Angiotensin System
  • Receptors, Adrenergic, beta
  • Radioimmunoassay
  • Propanolamines
  • Phenylephrine
  • Isoproterenol
  • Endothelium, Vascular
  • Culture Media
  • Chromatography, High Pressure Liquid
  • Cells, Cultured
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Tang, S. S., Stevenson, L., & Dzau, V. J. (1990). Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion. Circ Res, 66(1), 103–108. https://doi.org/10.1161/01.res.66.1.103
Tang, S. S., L. Stevenson, and V. J. Dzau. “Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion.Circ Res 66, no. 1 (January 1990): 103–8. https://doi.org/10.1161/01.res.66.1.103.
Tang SS, Stevenson L, Dzau VJ. Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion. Circ Res. 1990 Jan;66(1):103–8.
Tang, S. S., et al. “Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion.Circ Res, vol. 66, no. 1, Jan. 1990, pp. 103–08. Pubmed, doi:10.1161/01.res.66.1.103.
Tang SS, Stevenson L, Dzau VJ. Endothelial renin-angiotensin pathway. Adrenergic regulation of angiotensin secretion. Circ Res. 1990 Jan;66(1):103–108.

Published In

Circ Res

DOI

ISSN

0009-7330

Publication Date

January 1990

Volume

66

Issue

1

Start / End Page

103 / 108

Location

United States

Related Subject Headings

  • Renin-Angiotensin System
  • Receptors, Adrenergic, beta
  • Radioimmunoassay
  • Propanolamines
  • Phenylephrine
  • Isoproterenol
  • Endothelium, Vascular
  • Culture Media
  • Chromatography, High Pressure Liquid
  • Cells, Cultured