The responses of atrial natriuretic factor concentrations to acute volume changes in conscious rats.

Journal Article (Journal Article)

A rapid and sensitive radioimmunoassay has been developed for measurements of atrial natriuretic factor (ANF) in rat plasma. The antiserum, raised to rat ANF (99-126), cross-reacts with rat ANF (103-123), ANF (103-125), ANF (103-126) but not with smaller fragments, human ANF (99-126), angiotensin II, bradykinin or vasopressin. The plasma ANF concentration is 181 +/- 24 pg/ml (N = 24) in the unstressed conscious rats (Charles River CD, male). The ANF immunoreactivity in the plasma extracts was verified by HPLC analysis, which displayed one major immunoreactive peak of ANF corresponding to rat ANF (99-126) and some smaller fragments. Intravenous injection of saline elevated circulating ANF, whereas acute volume depletion by hemorrhage, water deprivation and furosemide diuresis greatly lowered plasma ANF. The prompt response of plasma ANF levels to acute changes in volume status is consistent with the proposed role of ANF as a volume-regulatory hormone.

Full Text

Duke Authors

Cited Authors

  • Chiu, PJ; Vemulapalli, S; Policelli, M; Kifor, I; Sybertz, EJ; Dzau, VJ

Published Date

  • November 23, 1987

Published In

Volume / Issue

  • 41 / 21

Start / End Page

  • 2339 - 2347

PubMed ID

  • 2960866

International Standard Serial Number (ISSN)

  • 0024-3205

Digital Object Identifier (DOI)

  • 10.1016/0024-3205(87)90657-6


  • eng

Conference Location

  • Netherlands