Endothelial renin-angiotensin pathway: evidence for intracellular synthesis and secretion of angiotensins.

Published

Journal Article

Cultured bovine aortic endothelial cells (BAEC) contain renin and angiotensinogen. To examine whether angiotensins are synthesized intracellularly and secreted by these cells, we assayed cell extracts as well as serum-free media of intact confluent BAEC. Angiotensins were identified by their retention time on reverse phase high performance liquid chromatography and direct radioimmunoassay. BAEC and their media contain angiotensin II and angiotensin III. The rate of angiotensin accumulation in the media was a nonlinear function of time; the highest rate occurred in the first 15 minutes. The amount of angiotensin II accumulated in 30 minutes exceeded 200% of the intracellular concentration and that of angiotensin III exceeded 500% of the initial intracellular content. Neither renin nor angiotensinogen could be detected in the media. The viability of these cells was supported by low lactic dehydrogenase activity in the media (less than 0.5% of cellular level). These data suggest that BAEC is capable of synthesizing and secreting angiotensins. We postulate that this endothelial-derived angiotensin system may play an important paracrine or autocrine role in influencing local vascular tone.

Full Text

Duke Authors

Cited Authors

  • Kifor, I; Dzau, VJ

Published Date

  • March 1, 1987

Published In

Volume / Issue

  • 60 / 3

Start / End Page

  • 422 - 428

PubMed ID

  • 3555883

Pubmed Central ID

  • 3555883

International Standard Serial Number (ISSN)

  • 0009-7330

Language

  • eng

Conference Location

  • United States