Healing of cultured human cornea after free electron laser ablation

Journal Article

Purpose. To study the healing process in cultured human corneas after Qswitched Er:YAG laser ablation. Methods. Fresh human cadaver corneas within were ablated with a Q-switched Er:YAG laser at 2.94 m wavelength. (100ns, 1 Hz) The radiant exposure was 500 mJ/cm2. A focal crater was created using between 60 and 80 pulses. A linear incision was also formed by slowly scanning the laser over the corneal surface. Each cornea was cultured on a tissue supporting frame immediately after the ablation. Culture media consisted of 92% minimum essential media, 8% fetal bovine serum, 0.125% HEPES buffer solution, 0.125% gentamicin, and 0.05% fungizone. The entire tissue frame and media container were kept in an incubator at 37°C and 5% C02. Serial macroscopic photographs of the cultured corneas were taken during the healing process. Histology was performed after 17 days of culture. Results. At 3 days, a transient haze was observed within the craters which then resolved between 7 and 14 days. No haze was seen along the linear incision. Histologie sections of the cornea showed complete re-epithelialization of the lased area. The endothelium appeared unaffected. Conclusions. Cultured human corneas may provide useful information regarding the healing process following laser ablation.

Duke Authors

Cited Authors

  • Robinson, RD; Shen, JH; Joos, KM; Shetlar, DJ; Edwards, GS; O'Day, DM

Published Date

  • 1997

Published In

Volume / Issue

  • 38 / 4

Start / End Page

  • S406 -

International Standard Serial Number (ISSN)

  • 0146-0404