Role of immersion refractometry for investigating laser-induced effects in cells.

Journal Article (Journal Article)

The broad background of scattered light observed in spectra of cell suspensions is reduced by factors of up to 20 by immersion refractometry allowing for improved spectroscopic determination of the absorption properties of cells in the 325-820 nm range. Refractive-index matched spectra of E. coli C1a exhibit a set of resonant features near 422, 561, and 582 nm. Exposure wavelengths are chosen based on this spectrum and cell viability is investigated in E. coli suspensions exposed to 350, 400, 422, 440, and 700 nm radiation delivered in nanosecond pulses with total doses from 500 millijoules to 60 Joules. We observe a loss in cell viability for doses greater than 1 Joule at 422 nm and for all doses at other wavelengths; exposures of less than 1 Joule at 422 nm enhance growth. Excluding exposures at wavelengths within the resonant feature, longer wavelengths are less effective at reducing the viability of E. coli C1a. This indicates the occurrence of at least two absorption processes.

Full Text

Duke Authors

Cited Authors

  • Tribble, J; Kozub, J; Aly, A; Ossoff, R; Edwards, G

Published Date

  • January 1, 1992

Published In

Volume / Issue

  • 12 / 4

Start / End Page

  • 459 - 463

PubMed ID

  • 1495374

Electronic International Standard Serial Number (EISSN)

  • 1096-9101

International Standard Serial Number (ISSN)

  • 0196-8092

Digital Object Identifier (DOI)

  • 10.1002/lsm.1900120416


  • eng