Neck-motor interactions trigger rotation of the kinesin stalk.

Published

Journal Article

Rotation of the coiled-coil stalk of the kinesin-14 motors is thought to drive displacements or steps by the motor along microtubules, but the structural changes that trigger stalk rotation and the nucleotide state in which it occurs are not certain. Here we report a kinesin-14 neck mutant that releases ADP more slowly than wild type and shows weaker microtubule affinity, consistent with defective stalk rotation. Unexpectedly, crystal structures show the stalk fully rotated - neck-motor interactions destabilize the stalk, causing it to rotate and ADP to be released, and alter motor affinity for microtubules. A new structural pathway accounts for the coupling of stalk rotation - the force-producing stroke - to changes in motor affinity for nucleotide and microtubules. Sequential disruption of salt bridges that stabilize the unrotated stalk could cause the stalk to initiate and complete rotation in different nucleotide states.

Full Text

Duke Authors

Cited Authors

  • Liu, H-L; Pemble, CW; Endow, SA

Published Date

  • 2012

Published In

Volume / Issue

  • 2 /

Start / End Page

  • 236 -

PubMed ID

  • 22355749

Pubmed Central ID

  • 22355749

Electronic International Standard Serial Number (EISSN)

  • 2045-2322

Digital Object Identifier (DOI)

  • 10.1038/srep00236

Language

  • eng

Conference Location

  • England