Determinants of kinesin motor polarity.
Journal Article (Journal Article)
The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.
- Endow, SA; Waligora, KW
- August 21, 1998
Volume / Issue
- 281 / 5380
Start / End Page
- 1200 - 1202
International Standard Serial Number (ISSN)
Digital Object Identifier (DOI)
- United States