Determinants of kinesin motor polarity.
Publication
, Journal Article
Endow, SA; Waligora, KW
Published in: Science
August 21, 1998
The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.
Duke Scholars
Published In
Science
DOI
ISSN
0036-8075
Publication Date
August 21, 1998
Volume
281
Issue
5380
Start / End Page
1200 / 1202
Location
United States
Related Subject Headings
- Recombinant Fusion Proteins
- Protein Structure, Secondary
- Mutation
- Molecular Sequence Data
- Microtubules
- Kinesins
- General Science & Technology
- Drosophila melanogaster
- Drosophila Proteins
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Endow, S. A., & Waligora, K. W. (1998). Determinants of kinesin motor polarity. Science, 281(5380), 1200–1202. https://doi.org/10.1126/science.281.5380.1200
Endow, S. A., and K. W. Waligora. “Determinants of kinesin motor polarity.” Science 281, no. 5380 (August 21, 1998): 1200–1202. https://doi.org/10.1126/science.281.5380.1200.
Endow SA, Waligora KW. Determinants of kinesin motor polarity. Science. 1998 Aug 21;281(5380):1200–2.
Endow, S. A., and K. W. Waligora. “Determinants of kinesin motor polarity.” Science, vol. 281, no. 5380, Aug. 1998, pp. 1200–02. Pubmed, doi:10.1126/science.281.5380.1200.
Endow SA, Waligora KW. Determinants of kinesin motor polarity. Science. 1998 Aug 21;281(5380):1200–1202.
Published In
Science
DOI
ISSN
0036-8075
Publication Date
August 21, 1998
Volume
281
Issue
5380
Start / End Page
1200 / 1202
Location
United States
Related Subject Headings
- Recombinant Fusion Proteins
- Protein Structure, Secondary
- Mutation
- Molecular Sequence Data
- Microtubules
- Kinesins
- General Science & Technology
- Drosophila melanogaster
- Drosophila Proteins
- Animals