Determinants of kinesin motor polarity.

Journal Article (Journal Article)

The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.

Full Text

Duke Authors

Cited Authors

  • Endow, SA; Waligora, KW

Published Date

  • August 21, 1998

Published In

Volume / Issue

  • 281 / 5380

Start / End Page

  • 1200 - 1202

PubMed ID

  • 9712586

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.281.5380.1200


  • eng

Conference Location

  • United States