Determinants of kinesin motor polarity.

Journal Article

The kinesin motor protein family members move along microtubules with characteristic polarity. Chimeric motors containing the stalk and neck of the minus-end-directed motor, Ncd, fused to the motor domain of plus-end-directed kinesin were analyzed. The Ncd stalk and neck reversed kinesin motor polarity, but mutation of the Ncd neck reverted the chimeric motor to plus-end movement. Thus, residues or regions contributing to motor polarity must be present in both the Ncd neck and the kinesin motor core. The neck-motor junction was critical for Ncd minus-end movement; attachment of the neck to the stalk may also play a role.

Full Text

Duke Authors

Cited Authors

  • Endow, SA; Waligora, KW

Published Date

  • August 21, 1998

Published In

Volume / Issue

  • 281 / 5380

Start / End Page

  • 1200 - 1202

PubMed ID

  • 9712586

International Standard Serial Number (ISSN)

  • 0036-8075

Language

  • eng

Conference Location

  • United States