Skip to main content

Separation of meiotic and mitotic effects of claret non-disjunctional on chromosome segregation in Drosophila.

Publication ,  Journal Article
Komma, DJ; Horne, AS; Endow, SA
Published in: EMBO J
February 1991

The claret (ca) locus in Drosophila encodes a kinesin-related motor molecule that is required for proper distribution of chromosomes in meiosis in females and in the early mitotic divisions of the embryo. Here we demonstrate that a mutant allele of claret non-disjunctional (ca(nd)), non-claret disjunctional Dominant (ncdD), causes abnormalities in meiotic chromosome segregation, but is near wild-type with respect to early mitotic chromosome segregation. DNA sequence analysis of this mutant allele reveals two missense mutations compared with the predicted wild-type protein. One mutation lies in a proposed microtubule binding region of the motor domain and affects an amino acid residue that is conserved in all kinesin-related proteins reported to date. This region of the motor domain can be used to distinguish meiotic and mitotic motor function, defining an amino acid sequence criterion for classifying motors according to function. ncdD's mutant meiotic effect, but near wild-type mitotic effect, suggests that interactions of the ca motor protein with spindle microtubules differ in meiosis and mitosis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

February 1991

Volume

10

Issue

2

Start / End Page

419 / 424

Location

England

Related Subject Headings

  • X Chromosome
  • Sequence Homology, Nucleic Acid
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Microtubule Proteins
  • Meiosis
  • Kinesins
  • Genes, Dominant
  • Drosophila
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Komma, D. J., Horne, A. S., & Endow, S. A. (1991). Separation of meiotic and mitotic effects of claret non-disjunctional on chromosome segregation in Drosophila. EMBO J, 10(2), 419–424. https://doi.org/10.1002/j.1460-2075.1991.tb07963.x
Komma, D. J., A. S. Horne, and S. A. Endow. “Separation of meiotic and mitotic effects of claret non-disjunctional on chromosome segregation in Drosophila.EMBO J 10, no. 2 (February 1991): 419–24. https://doi.org/10.1002/j.1460-2075.1991.tb07963.x.
Komma, D. J., et al. “Separation of meiotic and mitotic effects of claret non-disjunctional on chromosome segregation in Drosophila.EMBO J, vol. 10, no. 2, Feb. 1991, pp. 419–24. Pubmed, doi:10.1002/j.1460-2075.1991.tb07963.x.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

February 1991

Volume

10

Issue

2

Start / End Page

419 / 424

Location

England

Related Subject Headings

  • X Chromosome
  • Sequence Homology, Nucleic Acid
  • Mutation
  • Molecular Sequence Data
  • Mitosis
  • Microtubule Proteins
  • Meiosis
  • Kinesins
  • Genes, Dominant
  • Drosophila