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Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation

Publication ,  Journal Article
Coussen, F; Choquet, D; Sheetz, MP; Erickson, HP
Published in: Journal of Cell Science
June 15, 2002

Previous studies have shown that small beads coated with FN7-10, a four-domain cell adhesion fragment of fibronectin, bind to cell surfaces and translocate rearward. Here we investigate whether soluble constructs containing two to five FN7-10 units might be sufficient for activity. We have produced a monomer, three forms of dimers, a trimer and a pentamer of FN7-10, on the end of spacer arms. These oligomers could bind small clusters of up to five integrins. Fluorescence microscopy showed that the trimer and pentamer bound strongly to the cell surface, and within 5 minutes were prominently localized to actin fiber bundles. Monomers and dimers showed only diffuse localization. Beads coated with a low concentration (probably one complex per bead) of trimer or pentamer showed prolonged binding and rearward translocation, presumably with the translocating actin cytskeleton. Beads containing monomer or dimer showed only brief binding and diffusive movements. We conclude that clusters of three integrin-binding ligands are necessary and sufficient for coupling to and translocating with the actin cytoskeleton.

Duke Scholars

Published In

Journal of Cell Science

ISSN

0021-9533

Publication Date

June 15, 2002

Volume

115

Issue

12

Start / End Page

2581 / 2590

Related Subject Headings

  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
  • 06 Biological Sciences
 

Citation

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Coussen, F., Choquet, D., Sheetz, M. P., & Erickson, H. P. (2002). Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation. Journal of Cell Science, 115(12), 2581–2590.
Coussen, F., D. Choquet, M. P. Sheetz, and H. P. Erickson. “Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation.” Journal of Cell Science 115, no. 12 (June 15, 2002): 2581–90.
Coussen F, Choquet D, Sheetz MP, Erickson HP. Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation. Journal of Cell Science. 2002 Jun 15;115(12):2581–90.
Coussen, F., et al. “Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation.” Journal of Cell Science, vol. 115, no. 12, June 2002, pp. 2581–90.
Coussen F, Choquet D, Sheetz MP, Erickson HP. Trimers of the fivronectin cell adhesion domain localize to actin filament bundles and undergo rearward translocation. Journal of Cell Science. 2002 Jun 15;115(12):2581–2590.
Journal cover image

Published In

Journal of Cell Science

ISSN

0021-9533

Publication Date

June 15, 2002

Volume

115

Issue

12

Start / End Page

2581 / 2590

Related Subject Headings

  • Developmental Biology
  • 3101 Biochemistry and cell biology
  • 11 Medical and Health Sciences
  • 06 Biological Sciences