Evidence for a junctional feet-ryanodine receptor complex from sarcoplasmic reticulum.

Journal Article (Journal Article)

Heavy sarcoplasmic reticulum vesicles, labelled with the Ca2+ release channel probe [3H]ryanodine, were solubilized in detergent, then centrifuged through sucrose gradients. A single peak of ryanodine binding activity was observed with an apparent sedimentation coefficient of 30S. Electron microscopy of the peak fraction showed disk structures of 25-28 nm diameter and 10 nm thickness. Proteins specifically enriched in the peak fraction were the Mr 160,000 and 260,000 and junctional feet proteins (Mr 320,000 and 300,000). This suggests that the feet proteins and ryanodine receptor may be specifically associated into a large oligomeric complex comprising subunits of Mr 160,000-320,000.

Full Text

Duke Authors

Cited Authors

  • Lai, FA; Erickson, H; Block, BA; Meissner, G

Published Date

  • March 13, 1987

Published In

Volume / Issue

  • 143 / 2

Start / End Page

  • 704 - 709

PubMed ID

  • 2436616

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(87)91411-2


  • eng

Conference Location

  • United States