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Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts.

Publication ,  Journal Article
Eroglu, C; Brugger, B; Wieland, F; Sinning, I
Published in: Proc Natl Acad Sci U S A
September 2, 2003

Metabotropic glutamate receptors (mGluRs) are responsible for the effects of glutamate in slow synaptic transmission, and are implicated in the regulation of many processes in the CNS. Recently, we have reported the expression and purification of a mGluR from Drosophila melanogaster (DmGluRA), a homologue of mammalian group II mGluRs. We have shown that ligand binding to reconstituted DmGluRA requires the presence of ergosterol in the liposomes [Eroglu, C., Cronet, P., Panneels, V., Beaufils, P. & Sinning, I. (2002) EMBO Rep. 3, 491-496]. Here we demonstrate that the receptor exists in different affinity states for glutamate, depending on the membrane composition. The receptor is in a high-affinity state when associated with sterol-rich lipid microdomains (rafts), and in a low-affinity state out of rafts. Enrichment of the membranes with cholesterol shifts the receptor into the high-affinity state, and induces its association with rafts. The receptor was crosslinked to photocholesterol. Our data suggest that sterol-rich lipid rafts act as positive allosteric regulators of DmGluRA.

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Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 2, 2003

Volume

100

Issue

18

Start / End Page

10219 / 10224

Location

United States

Related Subject Headings

  • Spodoptera
  • Receptors, Metabotropic Glutamate
  • Membrane Microdomains
  • Glutamic Acid
  • Drosophila melanogaster
  • Cholesterol
  • Animals
  • Allosteric Regulation
 

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Eroglu, C., Brugger, B., Wieland, F., & Sinning, I. (2003). Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc Natl Acad Sci U S A, 100(18), 10219–10224. https://doi.org/10.1073/pnas.1737042100
Eroglu, Cagla, Britta Brugger, Felix Wieland, and Irmgard Sinning. “Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts.Proc Natl Acad Sci U S A 100, no. 18 (September 2, 2003): 10219–24. https://doi.org/10.1073/pnas.1737042100.
Eroglu C, Brugger B, Wieland F, Sinning I. Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10219–24.
Eroglu, Cagla, et al. “Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts.Proc Natl Acad Sci U S A, vol. 100, no. 18, Sept. 2003, pp. 10219–24. Pubmed, doi:10.1073/pnas.1737042100.
Eroglu C, Brugger B, Wieland F, Sinning I. Glutamate-binding affinity of Drosophila metabotropic glutamate receptor is modulated by association with lipid rafts. Proc Natl Acad Sci U S A. 2003 Sep 2;100(18):10219–10224.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

September 2, 2003

Volume

100

Issue

18

Start / End Page

10219 / 10224

Location

United States

Related Subject Headings

  • Spodoptera
  • Receptors, Metabotropic Glutamate
  • Membrane Microdomains
  • Glutamic Acid
  • Drosophila melanogaster
  • Cholesterol
  • Animals
  • Allosteric Regulation