Automating Procedures for Processing, Cryopreservation, Storage, and Manipulation of Human Peripheral Blood Mononuclear Cells


Journal Article

Cell-mediated immune responses have been found to play a crucial role in the protection from, or amelioration of, many human diseases. There are many vaccine trials being conducted where the most important immune assessment consists of measuring T cell responses. The most readily accessible source of T cells is the peripheral blood. Thus, collection, processing, cryopreservation, storage, and manipulation of human peripheral blood mononuclear cells (PBMCs) are all key steps for assessment of vaccine and disease-induced immune responses. Automation of these procedures is not currently practiced widely. This article reviews current practices and explores the need for automation. © 2004, Society for Laboratory Automation and Screening. All rights reserved.

Full Text

Duke Authors

Cited Authors

  • Cox, JH; Ferrari, G; Bailer, RT; Koup, RA

Published Date

  • January 1, 2004

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 16 - 23

Electronic International Standard Serial Number (EISSN)

  • 1540-2452

International Standard Serial Number (ISSN)

  • 2211-0682

Digital Object Identifier (DOI)

  • 10.1016/S1535-5535-03-00202-8

Citation Source

  • Scopus