Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.


Journal Article

Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.

Full Text

Duke Authors

Cited Authors

  • Wilson, JW; Degan, S; Warren, WS; Fischer, MC

Published Date

  • November 2012

Published In

Volume / Issue

  • 3 / 11

Start / End Page

  • 2752 - 2760

PubMed ID

  • 23162713

Pubmed Central ID

  • 23162713

Electronic International Standard Serial Number (EISSN)

  • 2156-7085

International Standard Serial Number (ISSN)

  • 2156-7085

Digital Object Identifier (DOI)

  • 10.1364/BOE.3.002752


  • eng