Optical clearing of archive-compatible paraffin embedded tissue for multiphoton microscopy.

Published

Journal Article

Standard histopathology techniques (including paraffin embedding) are incompatible with thick tissue multiphoton imaging, and standard clearing techniques on those specimens destroy some molecular information. We demonstrate multiphoton imaging in specimens prepared according to standard histopathology techniques. This permits unlabeled 3-dimensional histology on archival tissue banks, which is of great value in evaluating prognostic indicators.

Full Text

Published version (via Digital Object Identifier)
Pubmed Central version

Duke Authors

Fischer, Martin
Warren, Warren S.

Cited Authors

Wilson, JW; Degan, S; Warren, WS; Fischer, MC

Published Date

November 2012

Published In

Biomedical Optics Express

Volume / Issue

3 / 11

Start / End Page

2752 - 2760

PubMed ID

23162713

Pubmed Central ID

23162713

Electronic International Standard Serial Number (EISSN)

2156-7085

International Standard Serial Number (ISSN)

2156-7085

Digital Object Identifier (DOI)

10.1364/BOE.3.002752

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