Deep tissue imaging approaches by direct capture of two-photon absorption

Conference Paper

Multiphoton imaging with fluorescence detection has proven to be a powerful method for moderately deep tissue imaging (less than 1 mm) with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or with low efficiency (Melanin). In this paper two femtosecond pulse shaping methods have been demonstrated to detect two-photon absorption (TPA), instead of fluorescence, with high sensitivity, which opens up a wide range of molecular targets. It also facilitates longer excitation wavelengths, which permit greater tissue penetration. Developing the imaging system by using these new methods will be also discussed. © 2004 IEEE.

Duke Authors

Cited Authors

  • Ye, T; Wagner, W; Tian, P; Yurtsever, G; Fischer, M; Warren, WS

Published Date

  • December 1, 2004

Published In

  • 2004 2nd Ieee International Symposium on Biomedical Imaging: Macro to Nano

Volume / Issue

  • 1 /

Start / End Page

  • 668 - 671

International Standard Book Number 10 (ISBN-10)

  • 0780383885

International Standard Book Number 13 (ISBN-13)

  • 9780780383883

Citation Source

  • Scopus