Deep tissue imaging approaches by direct capture of two-photon absorption
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, Conference
Ye, T; Wagner, W; Tian, P; Yurtsever, G; Fischer, M; Warren, WS
Published in: 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
December 1, 2004
Multiphoton imaging with fluorescence detection has proven to be a powerful method for moderately deep tissue imaging (less than 1 mm) with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or with low efficiency (Melanin). In this paper two femtosecond pulse shaping methods have been demonstrated to detect two-photon absorption (TPA), instead of fluorescence, with high sensitivity, which opens up a wide range of molecular targets. It also facilitates longer excitation wavelengths, which permit greater tissue penetration. Developing the imaging system by using these new methods will be also discussed. © 2004 IEEE.
Duke Scholars
Published In
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
ISBN
9780780383883
Publication Date
December 1, 2004
Volume
1
Start / End Page
668 / 671
Citation
APA
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ICMJE
MLA
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Ye, T., Wagner, W., Tian, P., Yurtsever, G., Fischer, M., & Warren, W. S. (2004). Deep tissue imaging approaches by direct capture of two-photon absorption. In 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano (Vol. 1, pp. 668–671).
Ye, T., W. Wagner, P. Tian, G. Yurtsever, M. Fischer, and W. S. Warren. “Deep tissue imaging approaches by direct capture of two-photon absorption.” In 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, 1:668–71, 2004.
Ye T, Wagner W, Tian P, Yurtsever G, Fischer M, Warren WS. Deep tissue imaging approaches by direct capture of two-photon absorption. In: 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004. p. 668–71.
Ye, T., et al. “Deep tissue imaging approaches by direct capture of two-photon absorption.” 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, vol. 1, 2004, pp. 668–71.
Ye T, Wagner W, Tian P, Yurtsever G, Fischer M, Warren WS. Deep tissue imaging approaches by direct capture of two-photon absorption. 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. 2004. p. 668–671.
Published In
2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
ISBN
9780780383883
Publication Date
December 1, 2004
Volume
1
Start / End Page
668 / 671