Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique
Publication
, Journal Article
Powell, KD; Fitzgerald, MC
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
A new MALDI-based technique, stability of unpurified proteins by rates of H/D exchange (SUPREX), for quantitative determination of protein-peptide binding constants was discussed. For the study the five different type of peptide sequence were used, which includes peptide #1, peptide #2, peptide #3, peptide #4 and peptide #5. The results show that phosphate may have a larger stabilizing effect on ligands with high K d values (weak binders) than on ligands with low K d values (strong binders). It was suggested that the S-protein binds to a number of peptide ligands.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
445 / 446
Citation
APA
Chicago
ICMJE
MLA
NLM
Powell, K. D., & Fitzgerald, M. C. (2002). Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 445–446.
Powell, K. D., and M. C. Fitzgerald. “Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 445–46.
Powell KD, Fitzgerald MC. Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;445–6.
Powell, K. D., and M. C. Fitzgerald. “Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 445–46.
Powell KD, Fitzgerald MC. Quantitative determination of protein-peptide binding constants using a new, MALDI- and H/D-exchange based technique. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;445–446.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
445 / 446