A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution
Publication
, Journal Article
Fitzgerald, MC; Powell, KD; Wang, MZ; Ma, L; Ghaemmaghami, S; Oas, TG
Published in: Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
December 1, 2002
The protein-ligand binding interactions in solution were investigated using mass spectrometry. The H/D exchange and mass spectrometry-based technique termed as stability of unpurified proteins by rates of H/D exchange (SUPREX) was developed to evaluate thermodynamic stability of proteins. The analysis was initiated by 10-fold dilution of stock solution of protein in series of deuterated exchange buffers which varied in concentration of deuterated guanidine. The results show that increased H/D time shifted the SUPREX curve transition midpoint to lower denaturant.
Duke Scholars
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
395 / 396
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Fitzgerald, M. C., Powell, K. D., Wang, M. Z., Ma, L., Ghaemmaghami, S., & Oas, T. G. (2002). A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, 395–396.
Fitzgerald, M. C., K. D. Powell, M. Z. Wang, L. Ma, S. Ghaemmaghami, and T. G. Oas. “A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, December 1, 2002, 395–96.
Fitzgerald MC, Powell KD, Wang MZ, Ma L, Ghaemmaghami S, Oas TG. A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;395–6.
Fitzgerald, M. C., et al. “A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution.” Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics, Dec. 2002, pp. 395–96.
Fitzgerald MC, Powell KD, Wang MZ, Ma L, Ghaemmaghami S, Oas TG. A general mass spectrometry-based method for the quantitation of protein-ligand binding interactions in solution. Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002 Dec 1;395–396.
Published In
Proceedings 50th ASMS Conference on Mass Spectrometry and Allied Topics
Publication Date
December 1, 2002
Start / End Page
395 / 396