Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.

Journal Article (Journal Article)

Techniques currently available to obtain anti-idiotypic reagents reactive with a single chain of a lymphocyte antigen receptor rely on immunization with intact soluble or cell-bound Ig or T-cell receptors. Ready recovery of single-chain-specific monoclonal antibodies (MAbs) depends on the presence of an immunodominant epitope on the desired chain and chance recovery of the responding clone. Here we present a method to maximize recovery of an Ig heavy-chain-specific anti-idiotypic Ig, using sequential immunization with MAbs expressing the H chain V region in combination with different H chain isotypes and with different light chains. The latter was produced by in vitro transfection of an H-chain-loss variant myeloma cell line with a transgene construct expressing the Ig H chain V region of interest. Sequential immunization may be a useful strategy to enhance selection of anti-Id reagents reactive with single chain-specific epitopes.

Full Text

Duke Authors

Cited Authors

  • Foster, MH; Cooperstone, BG; Chen, H

Published Date

  • August 1998

Published In

Volume / Issue

  • 17 / 4

Start / End Page

  • 323 - 329

PubMed ID

  • 9790066

International Standard Serial Number (ISSN)

  • 0272-457X

Digital Object Identifier (DOI)

  • 10.1089/hyb.1998.17.323


  • eng

Conference Location

  • United States