Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.
Journal Article (Journal Article)
Techniques currently available to obtain anti-idiotypic reagents reactive with a single chain of a lymphocyte antigen receptor rely on immunization with intact soluble or cell-bound Ig or T-cell receptors. Ready recovery of single-chain-specific monoclonal antibodies (MAbs) depends on the presence of an immunodominant epitope on the desired chain and chance recovery of the responding clone. Here we present a method to maximize recovery of an Ig heavy-chain-specific anti-idiotypic Ig, using sequential immunization with MAbs expressing the H chain V region in combination with different H chain isotypes and with different light chains. The latter was produced by in vitro transfection of an H-chain-loss variant myeloma cell line with a transgene construct expressing the Ig H chain V region of interest. Sequential immunization may be a useful strategy to enhance selection of anti-Id reagents reactive with single chain-specific epitopes.
Full Text
Duke Authors
Cited Authors
- Foster, MH; Cooperstone, BG; Chen, H
Published Date
- August 1998
Published In
Volume / Issue
- 17 / 4
Start / End Page
- 323 - 329
PubMed ID
- 9790066
International Standard Serial Number (ISSN)
- 0272-457X
Digital Object Identifier (DOI)
- 10.1089/hyb.1998.17.323
Language
- eng
Conference Location
- United States