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Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.

Publication ,  Journal Article
Foster, MH; Cooperstone, BG; Chen, H
Published in: Hybridoma
August 1998

Techniques currently available to obtain anti-idiotypic reagents reactive with a single chain of a lymphocyte antigen receptor rely on immunization with intact soluble or cell-bound Ig or T-cell receptors. Ready recovery of single-chain-specific monoclonal antibodies (MAbs) depends on the presence of an immunodominant epitope on the desired chain and chance recovery of the responding clone. Here we present a method to maximize recovery of an Ig heavy-chain-specific anti-idiotypic Ig, using sequential immunization with MAbs expressing the H chain V region in combination with different H chain isotypes and with different light chains. The latter was produced by in vitro transfection of an H-chain-loss variant myeloma cell line with a transgene construct expressing the Ig H chain V region of interest. Sequential immunization may be a useful strategy to enhance selection of anti-Id reagents reactive with single chain-specific epitopes.

Duke Scholars

Published In

Hybridoma

DOI

ISSN

0272-457X

Publication Date

August 1998

Volume

17

Issue

4

Start / End Page

323 / 329

Location

United States

Related Subject Headings

  • Rats
  • Mice, Transgenic
  • Mice
  • Laminin
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Epitopes
  • Antibody Specificity
  • Antibodies, Monoclonal
  • Antibodies, Anti-Idiotypic
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Foster, M. H., Cooperstone, B. G., & Chen, H. (1998). Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region. Hybridoma, 17(4), 323–329. https://doi.org/10.1089/hyb.1998.17.323
Foster, M. H., B. G. Cooperstone, and H. Chen. “Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.Hybridoma 17, no. 4 (August 1998): 323–29. https://doi.org/10.1089/hyb.1998.17.323.
Foster MH, Cooperstone BG, Chen H. Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region. Hybridoma. 1998 Aug;17(4):323–9.
Foster, M. H., et al. “Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region.Hybridoma, vol. 17, no. 4, Aug. 1998, pp. 323–29. Pubmed, doi:10.1089/hyb.1998.17.323.
Foster MH, Cooperstone BG, Chen H. Anti-idiotypic monoclonal Ig specific for an anti-laminin Ig heavy chain transgene variable region. Hybridoma. 1998 Aug;17(4):323–329.

Published In

Hybridoma

DOI

ISSN

0272-457X

Publication Date

August 1998

Volume

17

Issue

4

Start / End Page

323 / 329

Location

United States

Related Subject Headings

  • Rats
  • Mice, Transgenic
  • Mice
  • Laminin
  • Immunoglobulin Variable Region
  • Immunoglobulin Heavy Chains
  • Epitopes
  • Antibody Specificity
  • Antibodies, Monoclonal
  • Antibodies, Anti-Idiotypic