Variable region sequence analysis of anti-DNA antibodies: evidence for a family of closely related germ-line VH genes encoding lupus autoantibodies.

Journal Article (Journal Article)

Cloning and sequencing of the V regions of the anti-DNA monoclonal antibodies (mAbs), H438 and H130, indicate that H438 is encoded by a J558 VH gene, a single D region nucleotide, and unmutated JH1, V kappa-1C and J kappa 1 genes, and the H130 L chain is encoded by a V kappa-21 subgroup gene J kappa 1 gene. Identification of VH438, which shared VH hybridization pattern with 6% of a panel of 352 MRL/lpr hybridomas, suggests that the frequency of J558 use among spontaneously activated B cells in MRL/lpr mice is greater than previously reported. The VHH438 J558 family gene is identical to VHPAR, which encodes the independently derived MRL/lpr autoantibody, MRP-2, and is highly homologous to the previously reported VHH130, which is identical to a BALB/c germ-line VH gene. Comparison of consensus sequences of homologous autoantibodies and previously reported restriction mapping suggest that a minimum of three highly related J558 germ-line genes encode lupus autoantibodies.

Full Text

Duke Authors

Cited Authors

  • Foster, MH; Madaio, MP; Barrett, KJ

Published Date

  • April 1992

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 175 - 182

PubMed ID

  • 1567551

Electronic International Standard Serial Number (EISSN)

  • 1557-7430

International Standard Serial Number (ISSN)

  • 1044-5498

Digital Object Identifier (DOI)

  • 10.1089/dna.1992.11.175


  • eng