Variable region sequence analysis of anti-DNA antibodies: evidence for a family of closely related germ-line VH genes encoding lupus autoantibodies.
Published
Journal Article
Cloning and sequencing of the V regions of the anti-DNA monoclonal antibodies (mAbs), H438 and H130, indicate that H438 is encoded by a J558 VH gene, a single D region nucleotide, and unmutated JH1, V kappa-1C and J kappa 1 genes, and the H130 L chain is encoded by a V kappa-21 subgroup gene J kappa 1 gene. Identification of VH438, which shared VH hybridization pattern with 6% of a panel of 352 MRL/lpr hybridomas, suggests that the frequency of J558 use among spontaneously activated B cells in MRL/lpr mice is greater than previously reported. The VHH438 J558 family gene is identical to VHPAR, which encodes the independently derived MRL/lpr autoantibody, MRP-2, and is highly homologous to the previously reported VHH130, which is identical to a BALB/c germ-line VH gene. Comparison of consensus sequences of homologous autoantibodies and previously reported restriction mapping suggest that a minimum of three highly related J558 germ-line genes encode lupus autoantibodies.
Full Text
Duke Authors
Cited Authors
- Foster, MH; Madaio, MP; Barrett, KJ
Published Date
- April 1992
Published In
Volume / Issue
- 11 / 3
Start / End Page
- 175 - 182
PubMed ID
- 1567551
Pubmed Central ID
- 1567551
International Standard Serial Number (ISSN)
- 1044-5498
Digital Object Identifier (DOI)
- 10.1089/dna.1992.11.175
Language
- eng
Conference Location
- United States